The study, led by the National Center for Atmospheric Research (NCAR), is being published this week in Science.
New work at Rockefeller University has revealed that one such channel in humans responds to mechanical force using a never-before-seen mechanism. Researchers…
A research team led by physicists at the University of Wisconsin-Milwaukee (UWM) has proven a method that makes it possible to find the atomic structure of…
Professor Hak-Sung Kim of the Department of Biological Sciences at Korea Advanced Institute of Science and Technology (KAIST) and Yiseul Ryu, a doctoral…
The record efficiency was achieved in outdoor tests in Sydney, before being independently confirmed by the National Renewable Energy Laboratory (NREL) at their…
The study suggests that attacking those subsets with targeted drugs may degrade the disease's ability to spread throughout the bone marrow of affected…
In vitro transcription/translation systems (ivtt) are used in biotechnology mainly to produce recombinant proteins whose production in vivo would be toxic. The experimental analysis normally involves autoradiography, i.e. it requires the labelling of translation products with radioactive isotopes. Handling these isotopes is expensive, a potential health hazard and limits throughput.
The present invention consists of stable bacterial strains with ribosomal subunits incorporating fluorescent markers, which have growth characteristics similar to wild type and which have an intact translation apparatus. This opens the opportunity for measuring the translation activity in real time using fluorescence. It is possible to carry out experiments in a shorter time and at less cost in multi-well plates, varying several reaction parameters in parallel to optimize yield.
The hierarchical and precisely controlled process creating ribosomes in living cells is known as ribosome assembly and is relatively little researched. In the eyes of many experts the early processes in the creation of ribosomes offer attractive targets for antimicrobial agents. The systematic search for such substances is made more difficult by the fact, that currently no suitable screening processes exist.
The present invention consists of stable bacterial strains with ribosomal subunits incorporating fluorescent markers, which have growth characteristics similar to wild type, and which have an intact translation apparatus. The positioning of the fluorophores allows for disturbances in the ribosome assembly to be detected in vivo by a fluorescence-based readout process. The process has been optimized for use with multi-well plates and thus is suitable for use in high throughput screenings (HTS).
Until recently, light trapping within periodically nanostructured solar cells could only be analysed using indirect methods, as captured light is not visible…
Further, scientists have observed that El Niño greatly influences the yearly variations of tropical cyclones (a general term which includes hurricanes,…
The Mainz chemistry professor Katja Heinze and her Luxembourg cooperation partner Dr. Patrick Choquet have received the Interregional Research Award for the…
In 2012, there were around 8.6 million cases of tuberculosis worldwide resulting in 1.3 million associated deaths according to the World Health Organisation…
Heidelberg researchers have gained new insights on the function of a presenilin-related protein in the model organism baker’s yeast with regard to the…
In the old days, it was the market square; today's generation of elderly people likes to have a chat at the café integrated into the supermarket. Stationary…
The immunologists Dr. Kristina Heinig and Dr. Uta Höpken (Max Delbrück Center for Molecular Medicine, MDC, Berlin-Buch) and the hematologist Dr. Armin Rehm…
Scientists of the Max-Born-Institut (MBI) in Berlin were able to show theoretically that the ultrafast x-ray camera is not only sensitive to inert core…
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