The present invention specifically provides a novel vaccine formulation suitable for ocular immunization and provides a method for inducing a local and systemic immune response. The combination of the three components antigen & corpusculate bodies (eg.Bortedella pertussis (wBP)) & adjuvans showed the highest rise in IgA antibody titers in tears and sera when compared to other immunization groups. Immunization via the conjunctiva with TTd plus wBP and adjuvant resulted in a 33% survival rate of challenged mice compared to a 0% survival rate in non-immunized animals.
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