According to the invention the approach of an easy risk assessment of patients with diagnosed cardiac insufficiency is based on the measurement of concentration of a pro-tein in whole blood called MD-2 by using the ELISA-technology. ELISA (Enzyme-Linked-Immunosorbent-Assay) is a common and standardized immunological assay for the detection of specific molecules in body liq-uids. It is well known that for cardiac degen-eration the activation of toll-like receptor-4 (TLR 4) is important. TLR 4 and MD-2 then form a receptor complex. By the additional use of MD-2 biomarker to the gold-standard biomarker BNP for risk stratification of cardiac insufficiency, an in-crease of sensitivity and specificity regarding to the long-term survival can be realized. The used biomarker for this assay, is a MD-2 protein (also called LY96) which relates to the group of so-called Immune response receptors.
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