One important biosynthetic precursor of fatty alcohols is hydroxyalkanoyloxy alkanoic acid (HAA). HAA can be produced by microbes using sugars as substrate. Currently, no commercial production of HAA is established in the art and only few literature exist that relates to HAA production. Due to their amphiphilic nature, HAA can be used as biosurfactant on one hand and as a precursor for the synthesis of alkanes, which can be used as fuel especially aviation fuel on the other hand. The present invention provides a novel procedure for a selective and quantitative (> 1g/L) production of HAAs from biotechnological process engineering. Industrial relevant strains like P. putida and E. coli have been tested and specific chain lengths of HAAs (short or long) can be obtained via direct product secretion into the media. Within one day, already 40% of theoretical product (in view of glucose as C-source) can be achieved. On behalf of the University of Aachen, PROvendis offers access to rights for commercial use as well as the opportunity for further co-development.
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