So far, the established methods for an efficient and cell-preserving transfection in high-throughput screening lead to unsatisfactory results. Within the scope of a project of the Industrial Joint Research (IFG), the Laser Zentrum Hannover e.V. (LZH) and its partners succeeded in developing a functional model for a gold nanoparticle-based laser transfection in high-throughput.
This transfection method is characterized by molecules entering the cells through an optically induced process. By attaching the gold nanoparticles to the cells, a photothermal effect is achieved under laser irradiation, which enables an efficient transfer of molecules into the cells.
Many advantages by changing the method
In comparison to the established methods for cell transfection, the risks and efforts can be considerably reduced by the gold nanoparticle process. The use of an optical mechanism also makes the method much more independent from the cell type and molecules used. Also, within larger samples, the transfection can be carried out both more precisely and cell-specifically.
Application tests and method comparisons for customers
Within the course of the project, a large amount of application data could already be collected, and application fields could be verified. The Biophotonic Imaging & Manipulation Group offers testing services for customer-specific methods and questions. Also, methods for cell transfection processes can be compared according to customer requirements.
Follow-up project with new partners
The project has been successfully completed by presenting a functional model. Now, the scientists are planning to continue their work. Methods for use in high-throughput testing shall be further developed and made ready for the market. Presently, the LZH is looking for partners from the screening area to build a prototype in the follow-up project.
The project “Setup and testing of a functional model for gold nanoparticle-based (GNOME) laser transfection in high throughput mode” is IGF project no. 18129 N of the Forschungsvereinigung Feinmechanik, Optik und Medizintechnik F.O.M. (Reseach Consortium Fine Mechanics, Optics and Medical Technology). It is supported via the German Federation of Industrial Research Associations "Otto von Guericke" e.V. (AiF) within the framewok of the Industrial Joint Research (IFG) of the Federal Ministry for Economic Affairs and Energy (BMWi). The members of the advisory committee were: Center for Applied Nanotechnology (CAN) GmbH, Cenix BioScience GmbH, European ScreeningPort GmbH, IBA GmbH, LaVision BioTec GmbH and LLS Rowiak LaserLabSolutions GmbH.
Dr. Nadine Tinne | Laser Zentrum Hannover e.V.
Intelligent wheelchairs, predictive prostheses
20.12.2017 | Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA
Jelly with memory – predicting the leveling of com-mercial paints
15.12.2017 | Fraunhofer-Institut für Produktionstechnik und Automatisierung IPA
Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.
But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...
Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.
The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
22.02.2018 | Life Sciences
22.02.2018 | Physics and Astronomy
22.02.2018 | Earth Sciences