So far, the established methods for an efficient and cell-preserving transfection in high-throughput screening lead to unsatisfactory results. Within the scope of a project of the Industrial Joint Research (IFG), the Laser Zentrum Hannover e.V. (LZH) and its partners succeeded in developing a functional model for a gold nanoparticle-based laser transfection in high-throughput.
This transfection method is characterized by molecules entering the cells through an optically induced process. By attaching the gold nanoparticles to the cells, a photothermal effect is achieved under laser irradiation, which enables an efficient transfer of molecules into the cells.
Many advantages by changing the method
In comparison to the established methods for cell transfection, the risks and efforts can be considerably reduced by the gold nanoparticle process. The use of an optical mechanism also makes the method much more independent from the cell type and molecules used. Also, within larger samples, the transfection can be carried out both more precisely and cell-specifically.
Application tests and method comparisons for customers
Within the course of the project, a large amount of application data could already be collected, and application fields could be verified. The Biophotonic Imaging & Manipulation Group offers testing services for customer-specific methods and questions. Also, methods for cell transfection processes can be compared according to customer requirements.
Follow-up project with new partners
The project has been successfully completed by presenting a functional model. Now, the scientists are planning to continue their work. Methods for use in high-throughput testing shall be further developed and made ready for the market. Presently, the LZH is looking for partners from the screening area to build a prototype in the follow-up project.
The project “Setup and testing of a functional model for gold nanoparticle-based (GNOME) laser transfection in high throughput mode” is IGF project no. 18129 N of the Forschungsvereinigung Feinmechanik, Optik und Medizintechnik F.O.M. (Reseach Consortium Fine Mechanics, Optics and Medical Technology). It is supported via the German Federation of Industrial Research Associations "Otto von Guericke" e.V. (AiF) within the framewok of the Industrial Joint Research (IFG) of the Federal Ministry for Economic Affairs and Energy (BMWi). The members of the advisory committee were: Center for Applied Nanotechnology (CAN) GmbH, Cenix BioScience GmbH, European ScreeningPort GmbH, IBA GmbH, LaVision BioTec GmbH and LLS Rowiak LaserLabSolutions GmbH.
Dr. Nadine Tinne | Laser Zentrum Hannover e.V.
New manufacturing process for SiC power devices opens market to more competition
14.09.2017 | North Carolina State University
Quick, Precise, but not Cold
17.05.2017 | Fraunhofer-Institut für Lasertechnik ILT
Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...
The Fraunhofer Institute for Laser Technology ILT and Rapid Shape GmbH are working together to further develop resin-based 3D printing. The new “TwoCure” process requires no support structures and is significantly more efficient and productive than conventional 3D printing techniques for plastic components. Experts from Fraunhofer ILT will be presenting the state-funded joint development that makes use of the interaction of light and cold in forming the components at formnext 2017 from November 14 to 17 in Frankfurt am Main.
Much like stereolithography, one of the best-known processes for printing 3D plastic components works using photolithographic light exposure that causes liquid...
A team of researchers led by Prof. Wolfram Pernice from the Institute of Physics at Münster University has developed a miniature abacus on a microchip which calculates using light signals. With it they are paving the way to the development of new types of computer in which, as in the human brain, the computing and storage functions are combined in one element.
Researchers at the universities of Münster, Exeter and Oxford have developed a miniature “abacus” which can be used for calculating with light signals. With it...
Extremely short electron bunches are key to many new applications including ultrafast electron microscopy and table-top free-electron lasers. A german team of physicists from Rostock University, the Max Born Institute in Berlin, the Ludwig-Maxmilians-Universität Munich, and the Max Planck Institute of Quantum Optics in Garching has now shown how electrons can be accelerated in an extreme and well-controlled way with laser light, while crossing a silver particle of just a few nanometers.
Of particular importance for potential applications is the ability to manipulate the acceleration process, known as a swing-by maneuver from space travel, with...
Cancer cells can reactivate a cellular process that is an essential part of embryonic development. This allows them to leave the primary tumor, penetrate the surrounding tissue and form metastases in peripheral organs. In the journal Nature Communications, researchers from the University of Basel’s Department of Biomedicine provide an insight into the molecular networks that regulate this process.
During an embryo’s development, epithelial cells can break away from the cell cluster, modify their cell type-specific properties, and migrate into other...
30.10.2017 | Event News
23.10.2017 | Event News
17.10.2017 | Event News
03.11.2017 | Physics and Astronomy
03.11.2017 | Earth Sciences
03.11.2017 | Physics and Astronomy