Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

PET imaging tracks Zika virus infection, disease progression in mouse model

20.09.2017

For the first time, scientists have used Positron Emission Tomography (PET) imaging to study brain inflammation following Zika virus infection in mice, according to a study recently published online in the journal Molecular Imaging and Biology. The technology has the added benefit of allowing investigators to study the progression of the disease over time.

Small-animal imaging has become an important research tool for preclinical studies of infectious diseases, according to senior author Thomas M. Bocan, Ph.D., of the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID).


Bottom row: The image shows representative PET/CT mouse brain images at days 3, 6 and 10 post-infection with Zika virus. Red area indicates neuroinflammation. Top row: Image represents PBS control mouse brain images.

Image courtesy of US Army Medical Research Institute of Infectious Diseases

"Traditional methods of infectious disease research using animal models have provided limited information about disease progression until the study's endpoint, when investigators could analyze tissues from those animals," he said. "Imaging studies allow us to gather enhanced information through longitudinal studies of the same animal during the course of the infection."

Zika virus was initially identified in monkeys in Uganda in 1947, with the first human case detected in 1952, according to the World Health Organization (WHO). The disease gained international attention in May 2015 when it began to spread throughout the Americas.

While the virus is primarily transmitted to people through the bite of an infected mosquito, sexual transmission is also possible. Zika virus infection during pregnancy has been linked to congenital brain abnormalities, including microcephaly, and Zika virus is a trigger of Guillain-Barré syndrome.

Continuing research to investigate the link between Zika virus and a range of neurological disorders requires a better understanding of the pathogenic mechanisms causing severe disease. PET is a highly sensitive molecular imaging technique that can be used to visualize a variety of biological processes in live animal models. The USAMRIID team therefore set out to evaluate the ability and sensitivity of PET imaging, using a probe called [18F]DPA-714 to detect and quantify neuroinflammation in Zika virus-infected mice.

As described in the paper, they found that levels of Zika virus in the mouse brain increased from day 3 to day 10 post-infection. Over this span, the mice also showed a 2- to 6-fold increase in global brain neuroinflammation using [18F]DPA-714 PET imaging.

The work highlights the significant role played by global neuroinflammation in the progression of Zika virus infection. In addition, it demonstrates, for the first time, the ability of [18F]DPA-714 PET imaging to detect and quantify Zika virus-related neuroinflammation disseminated throughout the brains of infected mice.

Noninvasive imaging using PET and other technologies reduces the number of animals in a study by permitting the use of animals as their own controls, according to the authors. In addition, therapeutic agents can be developed and tested using imaging technologies that are directly translatable to the clinic.

"The future is bright for the application of imaging in infectious diseases," said Bocan. "Measures of virus and bacteria distribution and the consequences of infection can be assessed in real time in the same subject. In addition, treatment with countermeasures can be evaluated with a better understanding of the state of disease progression."

###

USAMRIID's mission is to provide leading edge medical capabilities to deter and defend against current and emerging biological threat agents. Research conducted at USAMRIID leads to medical solutions--vaccines, drugs, diagnostics, and information--that benefit both military personnel and civilians. The Institute plays a key role as the lead military medical research laboratory for the Defense Threat Reduction Agency's Joint Science and Technology Office for Chemical and Biological Defense. USAMRIID is a subordinate laboratory of the U.S. Army Medical Research and Materiel Command. For more information, visit http://www.usamriid.army.mil.

Reference: [18F]DPA-714 PET imaging reveals global neuroinflammation in Zika virus infected mice. Kyle Kuszpit, Bradley S. Hollidge, Xiankun Zeng, Robert G. Stafford, Sharon Daye, Xiang Zhang, Falguni Basuli, Joseph W. Golden, Rolf E. Swenson, Darci R. Smith, Thomas M. Bocan. Molecular Imaging and Biology. DOI: 10.1007/s11307-017-1118-2

Funding: This study was supported by the Defense Advanced Research Projects Agency (DARPA).

Media Contact

Caree Vander Linden
teresa.l.vanderlinden.civ@mail.mil
301-619-2285

http://www.usamriid.army.mil 

Caree Vander Linden | EurekAlert!

More articles from Health and Medicine:

nachricht Researchers release the brakes on the immune system
18.10.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht Norovirus evades immune system by hiding out in rare gut cells
12.10.2017 | University of Pennsylvania School of Medicine

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

New procedure enables cultivation of human brain sections in the petri dish

19.10.2017 | Life Sciences

The “everywhere” protein: honour for the unravellor of its biology

19.10.2017 | Life Sciences

Rapid environmental change makes species more vulnerable to extinction

19.10.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>