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Expression and interaction of fluorescently labelled proteins makes living cells glow in different colours

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08.03.2007

A novel assay allows simultaneous detection of individual proteins and their interactions in living cells

Protein interactions direct cellular functions and their responses to pathogens and are important therapeutic targets. Scientists from the GSF Research Centre for Environment and Health have recently developed a method enabling simultaneous visualization of individual proteins and their interactions in living cells.

This is achieved by engineering the proteins to constantly emit red or blue fluorescent signals and to produce an additional yellow fluorescent signal upon interaction (see image below). Dr. Ruth Brack-Werner, Director of the GSF Institute of Molecular Virology (IMV) explains the decisive advantage of the new approach: “ In previous assays, signals were generated only by interacting proteins, whereas the individual partners remained undetected. However, the absence of signals could not be used to rule out protein interactions since the absence of one or both interaction partners would have the same effect. To overcome this problem Brack-Werner and her team developed the so-called extended bimolecular fluorescence complementation (exBiFC) which allows simultaneous monitoring of individual proteins and their interactions.

Brack-Werner and her colleagues’ groundbreaking research work focusses on mechanisms that control replication of the human immunodeficiency virus (HIV), which causes AIDS. “HIV replication is based on the interaction of cellular proteins with viral proteins. Interactions involving viral regulatory factors have a direct impact on the amount of virus produced by the HIV host cell”, Brack-Werner explains. “Preventing HIV proteins from interacting with their crucial partners is a promising approach to developing novel therapies.”

Therefore the GSF-scientists developed and validated exBiFC with the HIV Rev protein, which is an accelerator of HIV production. Various assays investigating Rev interactions in artificial settings indicate that the activity of Rev depends on the interaction of Rev molecules with each other and with cellular proteins. The latter include Exportin 1, which transports proteins from the nucleus to the cytoplasm and RISP, a modulator of HIV gene expression discovered by the Brack-Werner team in previous studies.

Brack-Werner and her team demonstrated that exBIFC allows visualization of interactions of Rev with itself and with Exportin1 and RISP in living cells. In addition they were able to compare the strengths of the interactions of Rev with its partners by analysing the intensities of the signals in cell images.

ExBiFC has a wide range of potential appllications and represents an important tool for the elucidation of protein interaction networks and discovery of novel antiviral factors. Thus exBIFC has an enormous potential in the battle against leading global health problems such as infectious diseases and cancers.

Michael van den Heuvel | Source: alphagalileo
Further information: www.gsf.de/neu/Aktuelles/Presse/2007/proteine_en.php

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