Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Watching the brain in action

13.10.2016

Watching millions of neurons in the brain interacting with each other is the ultimate dream of neuroscientists! A new imaging method now makes it possible to observe the activation of large neural circuits, currently up to the size of a small-animal brain, in real time and three dimensions. Researchers at the Helmholtz Zentrum München and the Technical University of Munich have recently reported on their new findings in Nature’s journal ‘Light: Science & Applications’.

Nowadays it is well recognized that most brain functions may not be comprehended through inspection of single neurons. To advance meaningfully, neuroscientists need the ability to monitor the activity of millions of neurons, both individually and collectively. However, such observations were so far not possible due to the limited penetration depth of optical microscopy techniques into a living brain.


The figure shows the fluorescence (left) and the FONT generated (right) image of a zebrafish brain. FONT allows high-resolution three-dimensional information of real-time neuronal activity (orange)

Source: Helmholtz Zentrum München

A team headed by Prof. Dr. Daniel Razansky, a group leader at the Institute of Biological and Molecular Imaging (IBMI), Helmholtz Zentrum München, and Professor of Molecular Imaging Engineering at the Technical University of Munich, has now found a way to address this challenge. The new method is based on the so-called optoacoustics*, which allows non-invasive interrogation of living tissues at centimeter scale depths.

”We discovered that optoacoustics can be made sensitive to the differences in calcium ion concentrations** resulting from neural activity and devised a rapid functional optoacoustic neuro-tomography (FONT) system that can simultaneously record signals from a very large number of neurons”, said Dr. Xosé Luis Deán-Ben, first author of the study. Experiments performed by the scientists in brains of adult zebrafish (Danio rerio) expressing genetically encoded calcium indicator GCaMP5G demonstrated, for the first time, the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in opaque brains. The technique was also able to trace neural activity during unrestrained motion of the animals.

Tracking the wildfire

"So far we demonstrated real-time analysis on whole brains of adult animals with roughly 2x3x4 millimeter dimensions (approximately 24 mm3)," says the study’s leader Razansky. State-of-the-art optical microscopy methods are currently limited to volumes well below a cubic millimeter when it comes to imaging of fast neural activity, according to the researchers. In addition, their FONT method is already capable of visualizing volumes of more than 1000 cubic millimeters with temporal resolution of 10 milliseconds.

Large-scale observation of neural activity is the key to understanding how the brain works, both under normal and diseased conditions. "Thanks to our method, one can now capture fast activity of millions of neurons simultaneously. Parallel neural networks with the social media: in the past, we were able to read along when someone (in this case, a nerve cell) placed a message with a neighbor. Now we can also see how this message spreads like wildfire," explains Razansky. "This new imaging tool is expected not only to significantly promote our knowledge on brain function and its pathophysiology but also accelerate development of novel therapies targeting neurological and neuropsychiatric disorders,” he concludes.

Further information

* Optoacoustics allows for high resolution, non-invasive, 3D imaging of living tissues. The technology uses short laser pulses that cause short-term expansion of the tissue, leading to tiny ultrasound vibrations. Those are registered with specially designed detectors, processed and converted into three-dimensional images of the interrogated tissue. To this end, the Helmholtz researchers have developed a number of optoacoustic imaging technologies for tracking hemodynamics and targeted agent delivery in a number of pre-clinical and medical imaging applications. The current work addresses significantly faster biological processes, such as neural activation.

** Nerve activation causes displacements of the calcium ions, which are transported in or out the neurons through so-called ion channels. Fluctuations in the intra-cellular calcium concentrations can be measured by means of genetically encoded calcium indicators (GECIs), which change their absorption spectrum, and consequently their color, depending on the amount of calcium present.

Background:
For his research, Razansky was awarded with highly competitive research grants from the European Research Council (ERC) and the US National Institutes of Health (NIH).
https://www.helmholtz-muenchen.de/en/press-media/press-releases/2016/press-relea...

Original Publication:
Deán-Ben, XL. et al. (2016): Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators. Light: Science & Applications, doi:10.1038/lsa.2016.201
http://aap.nature-lsa.cn:8080/cms/accessory/files/AAP-lsa2016201.pdf

The Helmholtz Zentrum München, the German Research Center for Environmental Health, pursues the goal of developing personalized medical approaches for the prevention and therapy of major common diseases such as diabetes and lung diseases. To achieve this, it investigates the interaction of genetics, environmental factors and lifestyle. The Helmholtz Zentrum München is headquartered in Neuherberg in the north of Munich and has about 2,300 staff members. It is a member of the Helmholtz Association, a community of 18 scientific-technical and medical-biological research centers with a total of about 37,000 staff members. http://www.helmholtz-muenchen.de/en

The Institute for Biological and Medical Imaging (IBMI) conducts research into in vivo imaging technologies for the biosciences. It develops systems, theories and methods of imaging and image reconstruction as well as animal models to test new technologies at the biological, preclinical and clinical level. The aim is to provide innovative tools for biomedical laboratories, for diagnosis and for the therapeutic monitoring of human diseases. http://www.helmholtz-muenchen.de/ibmi

Technical University of Munich (TUM) is one of Europe’s leading research universities, with more than 500 professors, around 10,000 academic and non-academic staff, and 39,000 students. Its focus areas are the engineering sciences, natural sciences, life sciences and medicine, reinforced by schools of management and education. TUM acts as an entrepreneurial university that promotes talents and creates value for society. In that it profits from having strong partners in science and industry. It is represented worldwide with a campus in Singapore as well as offices in Beijing, Brussels, Cairo, Mumbai, San Francisco, and São Paulo. Nobel Prize winners and inventors such as Rudolf Diesel, Carl von Linde, and Rudolf Mößbauer have done research at TUM. In 2006 and 2012 it won recognition as a German "Excellence University." In international rankings, TUM regularly places among the best universities in Germany. http://www.tum.de/en/homepage

Contact for the media:
Department of Communication, Helmholtz Zentrum München - German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg - Tel. +49 89 3187 2238 - Fax: +49 89 3187 3324 - E-mail: presse@helmholtz-muenchen.de

Scientific Contact at Helmholtz Zentrum München:
Prof. Dr. Daniel Razansky, Helmholtz Zentrum München - German Research Center for Environmental Health, Institute for Biological and Medical Imaging, Ingolstädter Landstr. 1, 85764 Neuherberg - Tel. +49 89 3187 1587, E-mail: daniel.razansky@helmholtz-muenchen.de

Weitere Informationen:

https://www.helmholtz-muenchen.de/en/press-media/press-releases/2016/index.html - Find more press releases of Helmholtz Zentrum München here

Sonja Opitz | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

Further reports about: Environmental Health TUM diseases neural activity neurons

More articles from Life Sciences:

nachricht A novel socio-ecological approach helps identifying suitable wolf habitats
17.02.2017 | Universität Zürich

nachricht New, ultra-flexible probes form reliable, scar-free integration with the brain
16.02.2017 | University of Texas at Austin

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Biocompatible 3-D tracking system has potential to improve robot-assisted surgery

17.02.2017 | Medical Engineering

Real-time MRI analysis powered by supercomputers

17.02.2017 | Medical Engineering

Antibiotic effective against drug-resistant bacteria in pediatric skin infections

17.02.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>