Chlamydia are the triggers for a whole range of serious diseases. At the same time, the bacteria are dependent for their survival on support from the cells they attack. The tricks they use here have been examined more closely by microbiologists from the University of Würzburg.
Bacteria of the Chlamydia trachomatis strain are responsible for a number of serious diseases in humans. A chlamydia infection is among the most common sexually transmitted diseases in the world. Current estimates suggest that, depending on the age group, up to ten percent of the population is infected with the pathogens worldwide.
Without treatment, these bacteria in women often cause the fallopian tubes to become blocked, which can result in ectopic pregnancies or infertility. Recent findings even indicate that chlamydia infections promote the development of ovarian cancer. Men can become infertile after infection.
A further consequence of a chlamydia infection arises mainly in tropical countries: there the bacteria attack the eyes and can lead to blindness. Around 150 million people are said to be suffering from this. Other strains can trigger pneumonia and are suspected of causing diseases such as atherosclerosis and Alzheimer’s disease.
The host looks after his guest
However, for chlamydia to be able to live and multiply, they are dependent on support from their “victim”. As the analysis of the Chlamydia trachomatis genome shows, the bacterium is entirely devoid of numerous metabolic processes and some exist only in fragments. For this reason, the bacterium needs to be supplied by its host cell with the necessary nutrients – nucleic acids, proteins, and lipids – continuously throughout its development cycle. It therefore has a strong interest in ensuring that the cell it has attacked stays intact and alive.
Professor Thomas Rudel, Chairman of the Department of Microbiology at the University of Würzburg, and his team have spent quite some time examining how chlamydia manage this. The scientists have now uncovered new details of the interaction between Chlamydia trachomatis and its host cell. They present their findings in the latest issue of Cell Reports.
Chlamydia prevent the cells from committing suicide
“When chlamydia attack a cell, this always causes considerable damage to the genetic material of this cell,” says Thomas Rudel. Normally, this would mean that the cell automatically “shuts down”, so to speak, or even initiates programmed cell death, known as apoptosis. This is how an organism prevents malfunctioning cells from multiplying in an uncontrolled manner and causing major damage. But in the case of a chlamydia infection this does not happen; it would appear that the bacterium is capable of preventing programmed cell death in the cell attacked.
“We were able to show that chlamydia deactivate the tumor suppressor protein p53 in the cells they have attacked and set in motion a process that ends up repairing the damage to the genetic material,” says Rudel. “Guardian of the genome”: the p53 protein is also known by this name. It has the ability to interrupt the cell cycle in damaged cells and, by doing so, to prevent the cell from dividing. This gives the cell more time to repair damage to the genetic material or to put itself out of action if it is beyond repair.
In a series of experiments, the Würzburg microbiologists were able to shed light on details of the relationship between the bacterium and the tumor suppressor. Their findings include the following:
• Since a chlamydia infection is always accompanied by damage to the genetic material, the bacteria prevent the tumor suppressor protein p53 from doing its job. In their experiments, the scientists did not manage even once to initiate cell death when administering high doses of a substance that is damaging to genetic material to cells attacked by chlamydia.
• If the researchers kept the concentration of p53 artificially high in infected cells, the chlamydia were no longer able to develop. They stayed at a stage in their lifecycle at which they are not infectious.
• How p53 keeps chlamydia in check is unclear. As it recently came to light that the protein influences a number of metabolic processes, including glycolysis and glucose transport, the Würzburg microbiologists also examined this aspect. After all, chlamydia are reliant on their host cell to supply them with glucose. However, a direct correlation could not be confirmed.
• On the other hand, the scientists were able to identify a mechanism elsewhere that enables the bacteria and the tumor suppressor protein to interact with one another: glucose-6-P-dehydrogenase – a key enzyme within the pentose phosphate cycle. If the researchers blocked this enzyme and, with it, the entire cycle, the growth of the chlamydia was curbed dramatically. However, if they stimulated the cycle, the bacteria continued to grow even if the concentration of p53 in the host cells was high.
“Our findings show two things very clearly. Firstly, chlamydia deactivate the tumor suppressor protein p53 so that they can actually multiply in the infected cell. The fact that they have to disable one of the main tumor suppressors to do this might explain the correlation between chlamydia infections and tumor development,” says Thomas Rudel. Secondly, the significance of the pentose phosphate cycle has been revealed – not just for repairing the genetic material, but also for optimally supplying the bacteria with vital nutrients.
Siegl et al., Tumor Suppressor p53 Alters Host Cell Metabolism to Limit Chlamydia trachomatis Infection, Cell Reports (2014), http://dx.doi.org/10.1016/j.celrep.2014.10.004
Prof. Dr. Thomas Rudel, Department of Microbiology, T +49 (0)931 31-84401, Thomas.Rudel@biozentrum.uni-wuerzburg.de
Gunnar Bartsch | idw - Informationsdienst Wissenschaft
More genes are active in high-performance maize
19.01.2018 | Rheinische Friedrich-Wilhelms-Universität Bonn
How plants see light
19.01.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau
On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.
We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...
What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...
For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.
Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...
At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.
No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...
Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.
Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...
08.01.2018 | Event News
11.12.2017 | Event News
08.12.2017 | Event News
19.01.2018 | Materials Sciences
19.01.2018 | Health and Medicine
19.01.2018 | Physics and Astronomy