Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The media is the message: How stem cells grow depends on what they grow up in

05.05.2015

Using mathematical model, UC San Diego researchers devise optimal human stem cell culture

Human pluripotent stem cells (hPSCs) possess the ability to grow into almost any kind of cell, which has made them dynamic tools for studying early human development and disease, but much depends upon what they grow up in.


This image shows colonies of human embryonic stem cells seen with a fluorescent microscope.

Credit: California Institute for Regenerative Medicine

Writing in the May 4 online issue of the journal Scientific Reports, researchers at University of California, San Diego School of Medicine used a powerful statistical tool called "design of experiments" or DOE to determine the optimal cell culture formula to grow and produce hPSCs.

"Currently, there are different culture methods and media that are not optimized or even chemically defined. There are several factors that may affect the growth of stem cells based on batch-to-batch media variation," said Alysson Muotri, PhD, associate professor in the UC San Diego departments of Pediatrics and Cellular and Molecular Medicine. "This affects science in many ways. For example, it slows down progress because conditions may not be pristine. It also makes it difficult for other labs to validate data because the media they use will likely not be the same as in the original experiments."

Muotri and colleagues used DOE to measure two critical growth factors used in hPSC media: basic fibroblast growth factor (bFGF) and neuregulin-1 beta 1 (NRG-1 beta 1). DOE is often used in scientific endeavors to measure and account for variations in data, but not so much in biology, said Muotri.

"If you ask a biology student what is the ideal temperature and pH for an enzyme, he/she will try to determine the best temperature in one experiment and the best pH in another experiment. Then, the student will erroneously conclude that these represent the optimal temperature and pH," said Muotri. "What is missing is the interaction between temperature and pH. The best working temperature may not be the most optimal pH condition. DOE takes into account positive, negative or neutral interactions between multiple factors at the same time."

Building upon earlier work, which had analyzed hundreds of other factors in hPSC media, the researchers determined the best formulations for bFGF and NRG-1 beta 1. They noted, however, that their findings are not fixed. "If science discovers a new factor that affects hPSC proliferation, we can add it into our DOE matrix to quickly test and re-formulate the media," said Muotri.

The researchers hope their findings will lead to a new standard for hPSC cultures. "Any lab in the world can have access to the same formulation, with no variability," said Muotri. "We also think this method could be applied towards the development of culture conditions during differentiation of human stem cells. Ideally, we want to create transition media formulations that subtly change during cell type specialization, mimicking the human embryo."

Muotri said his team is working with the UC San Diego Technology Transfer Office to find industry partners to assist in making the new technology accessible to all laboratories using hPSCs.

###

Co-authors include Paulo A. Marinho and Thanathom Chailangkarn, UCSD Department of Pediatrics/Rady Children's Hospital-San Diego, Department of Cellular and Molecular Medicine and UCSD Stem Cell Program.

Funding for this research came, in part, from the California Institute for Regenerative Medicine and the National Institutes of Health (1-DP2-OD006495-1).

Media Contact

Scott LaFee
slafee@ucsd.edu
619-543-6163

 @UCSanDiego

http://www.ucsd.edu 

Scott LaFee | EurekAlert!

Further reports about: Cellular Department Medicine Molecular Muotri UCSD University of California beta stem cells temperature

More articles from Life Sciences:

nachricht Scientists unlock ability to generate new sensory hair cells
22.02.2017 | Brigham and Women's Hospital

nachricht New insights into the information processing of motor neurons
22.02.2017 | Max Planck Florida Institute for Neuroscience

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Microhotplates for a smart gas sensor

22.02.2017 | Power and Electrical Engineering

Scientists unlock ability to generate new sensory hair cells

22.02.2017 | Life Sciences

Prediction: More gas-giants will be found orbiting Sun-like stars

22.02.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>