Scientists from the University of Würzburg give fascinating 3D-insights into the bone marrow, and successfully elucidated new details about the process of thrombocyte generation. These important findings could contribute to optimized therapeutic approaches for patients with bleeding disorders.
Thrombocytes, also known as platelets, play an important role in wound healing and tissue repair at sites of vascular damage by facilitating blood coagulation. As they possess only a short lifespan, new thrombocytes need to be constantly generated in the body. Platelets originate from the bone marrow where giant precursor cells, so-called megakaryocytes, undergo a complex maturation process and finally release platelets into the bloodstream.
Light Sheet Fluorescence Microscopy (LSFM): Reconstruction of a sternum bone (detailed view). Megakaryocytes (green) are mostly attached to vessels (red). Bone structure is depicted in grey.
Photo: Heinze Group
Light Sheet Fluorescence Microscopy (LSFM): Reconstruction of a murine femur bone (overview). Megakaryocytes (green) embedded into a dense vessel network (red) and the bone (grey).
Photo: Heinze Group
In the nursery of the thrombocytes it is calmer than one thought
So far, these precursor cells have been believed to migrate through the bone marrow to reach the vessel. By applying a combination of modern microscopy methods and computer simulations, researchers from the Rudolf Virchow Center for Experimental Biomedicine and the University Hospital of Würzburg were now able to disprove this theory.
The team of Prof. Katrin Heinze and Dr. David Stegner could show that most megakaryocytes already originate from the vascular niche, that is in close proximity to the vessel. The remaining precursor cells are uniformly distributed in the bone marrow and are typically so large and flexible in shape that they can reach the vessel by small (wobble) movements in the dense vessel network of the bone marrow.
"Only the combination of all information from intravital and light-sheet microscopy has made these findings possible," says Professor Katrin Heinze, director of the study. She further explains: "In addition, we were able to show how the 3D images of vessels and cells become perfect biological templates for realistic, eye-opening simulations of cell distributions in the bone marrow".
Complementary microscopy methods allow a virtual journey through the bone marrow
Dr. David Stegner, corresponding author of the new study, adds: "We have long suspected that the existing models of thrombopoiesis are insufficient or false, but it was difficult to prove this experimentally. This has now been achieved by high-resolution insights into the intact bone!“ The two leaders of the study point out that their collaboration was not only a stroke of luck, but a future-oriented decision that will continue for a long time to come.
"Based on our findings, new treatment strategies for diseases that are associated with reduced platelet formation could be developed in the future", hopes Dr. Stegner. The researchers from Würzburg recently published their new findings in the scientific journal Nature Communications.
Stegner, D. et al. (2017). Thrombopoiesis is spatially regulated by the bone marrow vasculature. Nature Communications 8(127). DOI: 10.1038/s41467-017-00201-7
Prof. Dr. Katrin Heinze (Molecular Microscopy, Rudolf Virchow Center)
Phone +49 (0)9 31/ 31 - 84214, email@example.com
Dr. David Stegner (group leader, Vascular Imaging)
Phone +49 (0)9 31/ 31 – 80419, firstname.lastname@example.org
Dr. Frank Sommerlandt (Public Science Center, Rudolf-Virchow-Zentrum)
Phone +49 (0)9 31/ 31 - 88449, email@example.com
Dr. Frank Sommerlandt | idw - Informationsdienst Wissenschaft
Could this protein protect people against coronary artery disease?
17.11.2017 | University of North Carolina Health Care
Microbial resident enables beetles to feed on a leafy diet
17.11.2017 | Max-Planck-Institut für chemische Ökologie
The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.
Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...
Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.
That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...
Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.
During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....
The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.
Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...
Pillared graphene would transfer heat better if the theoretical material had a few asymmetric junctions that caused wrinkles, according to Rice University...
15.11.2017 | Event News
15.11.2017 | Event News
30.10.2017 | Event News
17.11.2017 | Physics and Astronomy
17.11.2017 | Health and Medicine
17.11.2017 | Studies and Analyses