Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Slight Differences – New Insights into the Regulation of Disease-Associated Genes

16.06.2015

Researchers of the Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, in collaboration with the National Heart Research Institute Singapore (NHRIS), have gained new insights into the regulation of disease-associated genes. They used a new technique that enables them to observe gene regulation at the level of protein production. They could thus capture more individual gene regulations than with traditional methods that only capture gene expression and transcription (Nature Communications, doi: 10.1038/ncomms8200)*.

When a gene is read, its blueprint for proteins encoded in the language of DNA is transcribed in the cell nucleus into RNA. “At this level, many but by far not all of the individual differences in gene regulation can be identified,” said Professor Norbert Hübner, senior author of the publication and head of the research group Genetics and Genomics of Cardiovascular Diseases at the MDC.

Together with Sebastian Schafer (MDC, NHRIS) and Eleonora Adami (MDC) as well as researchers from several research institutions in Berlin, the Netherlands, England and the Czech Republic, they investigated gene regulation on the next level, translation. It takes place outside the cell nucleus, in the cell plasma. During translation, the RNA sequence is translated into amino acid sequences and assembled into proteins in the protein factories of the cell, the ribosomes.

First, the researchers searched the entire genome of two strains of rats, – one strain had high blood pressure, the other strain not – and specifically investigated genes of the heart and liver tissue. Then they used a new technique called ribosome profiling, abbreviated ribo-seq, which enables them to determine what proportion of the transcriptome is actively translated into proteins.

The result: They observed almost double the number of differentially expressed heart and liver genes in translation as in transcription. Next, they compared these data with the corresponding human genes in genome-wide association studies.

This comparison revealed that a large number of heart and liver genes in humans are regulated primarily during translation. The researchers are confident that capturing interindividual differences in the translated genome will lead to new insights into the genes and regulatory pathways underlying disease.

*Translational regulation shapes the molecular landscape of complex disease phenotypes
Sebastian Schafer1,2,*, Eleonora Adami1,*, Matthias Heinig1,3, Katharina E. Costa Rodrigues1, Franziska Kreuchwig1, Jan Silhavy4, Sebastiaan van Heesch1, Deimante Simaite1, Nikolaus Rajewsky5,6, Edwin Cuppen7, Michal Pravenec4, Martin Vingron3, Stuart A. Cook2,8,9 & Norbert Hübner1,6,10
1Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
2National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore, Singapore 169609, Singapore.
3Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany.
4Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenska 1083, 142 20 Prague 4, Czech Republic.
5Systems Biology of Gene Regulatory Elements, Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
6DZHK (German Centre for Cardiovascular Research), Partner Site, 13347 Berlin, Germany.
7Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
8National Heart and Lung Institute, Imperial College London, London SW3 6NP, UK.
9Duke-National University of Singapore, Singapore 169857, Singapore.
10Charité Universitätsmedizin, 10117 Berlin, Germany.
* These authors contributed equally to this work.

Contact:
Barbara Bachtler
Press Department
Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch
in the Helmholtz Association
Robert-Rössle-Straße 10
13125 Berlin
Germany
Phone: +49 (0) 30 94 06 - 38 96
Fax: +49 (0) 30 94 06 - 38 33
e-mail: presse@mdc-berlin.de
https://www.mdc-berlin.de/en

Weitere Informationen:

https://www.mdc-berlin.de/44659109/en/news/2015/20150616-slight_differences___ne...

Barbara Bachtler | Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft

More articles from Life Sciences:

nachricht How brains surrender to sleep
23.06.2017 | IMP - Forschungsinstitut für Molekulare Pathologie GmbH

nachricht A new technique isolates neuronal activity during memory consolidation
22.06.2017 | Spanish National Research Council (CSIC)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Quantum thermometer or optical refrigerator?

23.06.2017 | Physics and Astronomy

A 100-year-old physics problem has been solved at EPFL

23.06.2017 | Physics and Astronomy

Equipping form with function

23.06.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>