Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Rice bioengineers develop method to grow 3-D bone matrix

04.10.2002


Researchers use flowing fluids to create mechanical stress needed for bone formation


Tissue engineering researchers in Rice’s J.W. Cox Laboratory for Biomedical Engineering have developed a new technique that allows bone-forming cells to build a porous, 3-D bony matrix that’s structurally similar to natural bone. This photograph from an electron microscope shows a pore that has formed in a 3-D bony matrix. Bone-forming cells are clearly visible lining the walls.



A new study by Rice University researchers indicates that bioengineers growing bone in the laboratory may be able to create the mechanical stimulation needed to grow bone outside the body.

One of the greatest challenges tissue engineers face in growing bone in the laboratory is recreating the conditions that occur inside the body. The recipe for growing healthy bones includes not only a precise biological mix -- bone cells called "osteoblasts" and several growth factors that osteoblasts use to build the mineralized matrix of bones -- but also mechanical stimulation. Astronauts whose bones become brittle after months in orbit are a testament to the importance that mechanical stress plays in bone growth. In orbit, their skeletons aren’t subject to the everyday stresses of gravity.


Tissue engineers at Rice placed bone marrow-derived osteoblasts from rats into centimeter-wide plexiglass chambers containing a thin stack of titanium fiber mesh. The samples were covered with a liquid growth medium -- a bath of chemicals that promotes bone growth -- and sealed in an incubator. After letting the cultures sit overnight -- to give the cells time to attach themselves to the mesh -- engineers pumped growth medium through the cultures for 16 days. Bone cultures were subjected to a range of three different flow rates to provide mechanical stimulation, and another set of cultures were grown in a motionless bath.

Results of the research appear in the current issue of the Proceedings of the National Academy of Sciences USA.

"Researchers have used fluid flow to stimulate bone growth before, but no one has looked at its effect on three-dimensional cultures that have been subjected to continuous stimulation for several days," said Tony Mikos, the John W. Cox Professor of Bioengineering. "We found that even the lowest flow rate produced a significant increase in the formation of mineralized bone. Moreover, the mineralized bone that formed in samples subjected to flow was thick and well-developed -- similar to what we find in natural bone --while the bone matrix formed by the static samples was thin and brittle."

Mikos said more studies are needed to determine the exact flow rate needed to produce the optimal amount of bone matrix with the optimal three-dimensional structure. For those who have lost a segment of bone to cancer or injury, the technology isn’t expected to result in clinical treatment options for several years. Ultimately, however, artificial bone could be substituted for donor tissue or surgical implants made of synthetic materials.



The research was sponsored by the National Institutes of Health and NASA.

The article, titled "Fluid Flow Increases Mineralized Matrix Deposition in 3D Perfusion Culture of Marrow Stromal Osteoblasts in a Dose-Dependent Manner," by G.N. Bancroft, V.I. Sikavitsas, J. van den Dolder, T.L. Sheffield, C.G. Ambrose, J.A. Jansen, and A.G. Mikos, appears in the Oct. 1 issue of Proceedings of the National Academy of Sciences

Jade Boyd | EurekAlert!
Further information:
http://chico.rice.edu/

More articles from Life Sciences:

nachricht New photocatalyst speeds up the conversion of carbon dioxide into chemical resources
29.05.2017 | DGIST (Daegu Gyeongbuk Institute of Science and Technology)

nachricht Copper hydroxide nanoparticles provide protection against toxic oxygen radicals in cigarette smoke
29.05.2017 | Johannes Gutenberg-Universität Mainz

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Strathclyde-led research develops world's highest gain high-power laser amplifier

The world's highest gain high power laser amplifier - by many orders of magnitude - has been developed in research led at the University of Strathclyde.

The researchers demonstrated the feasibility of using plasma to amplify short laser pulses of picojoule-level energy up to 100 millijoules, which is a 'gain'...

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

New insights into the ancestors of all complex life

29.05.2017 | Earth Sciences

New photocatalyst speeds up the conversion of carbon dioxide into chemical resources

29.05.2017 | Life Sciences

NASA's SDO sees partial eclipse in space

29.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>