Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Rice bioengineers develop method to grow 3-D bone matrix

04.10.2002


Researchers use flowing fluids to create mechanical stress needed for bone formation


Tissue engineering researchers in Rice’s J.W. Cox Laboratory for Biomedical Engineering have developed a new technique that allows bone-forming cells to build a porous, 3-D bony matrix that’s structurally similar to natural bone. This photograph from an electron microscope shows a pore that has formed in a 3-D bony matrix. Bone-forming cells are clearly visible lining the walls.



A new study by Rice University researchers indicates that bioengineers growing bone in the laboratory may be able to create the mechanical stimulation needed to grow bone outside the body.

One of the greatest challenges tissue engineers face in growing bone in the laboratory is recreating the conditions that occur inside the body. The recipe for growing healthy bones includes not only a precise biological mix -- bone cells called "osteoblasts" and several growth factors that osteoblasts use to build the mineralized matrix of bones -- but also mechanical stimulation. Astronauts whose bones become brittle after months in orbit are a testament to the importance that mechanical stress plays in bone growth. In orbit, their skeletons aren’t subject to the everyday stresses of gravity.


Tissue engineers at Rice placed bone marrow-derived osteoblasts from rats into centimeter-wide plexiglass chambers containing a thin stack of titanium fiber mesh. The samples were covered with a liquid growth medium -- a bath of chemicals that promotes bone growth -- and sealed in an incubator. After letting the cultures sit overnight -- to give the cells time to attach themselves to the mesh -- engineers pumped growth medium through the cultures for 16 days. Bone cultures were subjected to a range of three different flow rates to provide mechanical stimulation, and another set of cultures were grown in a motionless bath.

Results of the research appear in the current issue of the Proceedings of the National Academy of Sciences USA.

"Researchers have used fluid flow to stimulate bone growth before, but no one has looked at its effect on three-dimensional cultures that have been subjected to continuous stimulation for several days," said Tony Mikos, the John W. Cox Professor of Bioengineering. "We found that even the lowest flow rate produced a significant increase in the formation of mineralized bone. Moreover, the mineralized bone that formed in samples subjected to flow was thick and well-developed -- similar to what we find in natural bone --while the bone matrix formed by the static samples was thin and brittle."

Mikos said more studies are needed to determine the exact flow rate needed to produce the optimal amount of bone matrix with the optimal three-dimensional structure. For those who have lost a segment of bone to cancer or injury, the technology isn’t expected to result in clinical treatment options for several years. Ultimately, however, artificial bone could be substituted for donor tissue or surgical implants made of synthetic materials.



The research was sponsored by the National Institutes of Health and NASA.

The article, titled "Fluid Flow Increases Mineralized Matrix Deposition in 3D Perfusion Culture of Marrow Stromal Osteoblasts in a Dose-Dependent Manner," by G.N. Bancroft, V.I. Sikavitsas, J. van den Dolder, T.L. Sheffield, C.G. Ambrose, J.A. Jansen, and A.G. Mikos, appears in the Oct. 1 issue of Proceedings of the National Academy of Sciences

Jade Boyd | EurekAlert!
Further information:
http://chico.rice.edu/

More articles from Life Sciences:

nachricht Symbiotic bacteria: from hitchhiker to beetle bodyguard
28.04.2017 | Johannes Gutenberg-Universität Mainz

nachricht Nose2Brain – Better Therapy for Multiple Sclerosis
28.04.2017 | Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Fighting drug resistant tuberculosis – InfectoGnostics meets MYCO-NET² partners in Peru

28.04.2017 | Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

 
Latest News

Wireless power can drive tiny electronic devices in the GI tract

28.04.2017 | Medical Engineering

Ice cave in Transylvania yields window into region's past

28.04.2017 | Earth Sciences

Nose2Brain – Better Therapy for Multiple Sclerosis

28.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>