Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Reading between the genes

03.06.2016

Our genes decide about many things in our lives – what we look like, which talents we have, or what kind of diseases we develop. For a long time dismissed as “junk DNA”, we now know that also the regions between the genes fulfil vital functions. They contain a complex control machinery with thousands of molecular switches that regulate the activity of our genes. Until now, however, regulatory DNA regions have been hard to find. Scientists around Patrick Cramer at the Max Planck Institute (MPI) for Biophysical Chemistry in Göttingen and Julien Gagneur at the Technical University of Munich (TUM) have now developed a method to find regulatory DNA regions which are active and controlling genes.

The genes in our DNA contain detailed assembly instructions for proteins, the “workers” carrying out and controlling virtually all processes in our cells. To ensure that each protein fulfils its tasks at the right time in the right place of our body, the activity of the corresponding gene has to be tightly controlled.


In contrast to older methods, TT-Seq (dark blue) allows scientists to gain a very consistent picture of all RNA molecules in the cell.

Margaux Michel, Patrick Cramer / Max Planck Institute for Biophysical Chemistry

This function is taken over by regulatory DNA regions between the genes, which act as a complex control machinery. “Regulatory DNA regions are essential for development in humans, tissue preservation, and the immune response, among others,” explains Patrick Cramer, head of the Department for Molecular Biology at the MPI for Biophysical Chemistry. “Furthermore, they play an important role in various diseases. For example, patients suffering from cancer or cardiovascular conditions show many mutations in exactly those DNA regions,” the biochemist says.

When regulatory DNA regions are active, they are first copied into RNA. “The resulting RNA molecules have a great disadvantage for us researchers though: The cell rapidly degrades them, thus they were hard to find until now,” reports Julien Gagneur, who recently moved with this group from the Gene Center of the Ludwig-Maximilians-Universität Munich to the TUM. “But exactly those short-lived RNA molecules often act as vital molecular switches that specifically activate genes needed in a certain place of our body. Without these molecular switches, our genes would not be functional.”

An anchor for short-lived molecular switches

Björn Schwalb and Margaux Michel, members of Cramer’s team, as well as Benedikt Zacher, scientist in Gagneur’s group, have now succeeded in developing a highly sensitive method to catch and identify even very short-lived RNA molecules – the so-called TT-Seq (transient transcriptome sequencing) method. The results are reported in the latest issue of the renowned scientific journal Science on June 3rd.

In order to catch the RNA molecules, the three junior researchers used a trick: They supplied cells with a molecule acting as a kind of anchor for a couple of minutes. The cells subsequently incorporated the anchor into each RNA they made during the course of the experiment. With the help of the anchor, the scientists were eventually able to fish the short-lived RNA molecules out of the cell and examine them.

“The RNA molecules we caught with the TT-Seq method provide a snapshot of all DNA regions that were active in the cell at a certain time – the genes as well as the regulatory regions between genes that were so hard to find until now,” Cramer explains. “With TT-Seq we now have a suitable tool to learn more about how genes are controlled in different cell types and how gene regulatory programs work,” Gagneur adds.

In many cases, researchers have a pretty good idea which genes play a role in a certain disease, but do not know which molecular switches are involved. The scientists around Cramer and Gagneur are hoping to be able to use the new method to uncover key mechanisms that play a role during the emergence or course of a disease. In a next step they want to apply their technique to blood cells to better understand the progress of a HIV infection in patients suffering from AIDS.

Original publication
Björn Schwalb, Margaux Michel, Benedikt Zacher, Katja Frühauf, Carina Demel, Achim Tresch, Julien Gagneur, Patrick Cramer: TT-Seq maps the human transient transcriptome.
Science 352,1225-1228 (2016), doi: 10.1126/science.aad9841.

Contact
Prof. Dr. Patrick Cramer, Department of Molecular Biology
Max Planck Institute for Biophysical Chemistry, Göttingen
Phone: +49 551 201-2800
E-mail: patrick.cramer@mpibpc.mpg.de

Prof. Dr. Julien Gagneur, Computational Biology Group
Technical University of Munich
Phone: +49 89 289-19411
E-mail: gagneur@in.tum.de

Dr. Anne Morbach, Public Relations Office
Max Planck Institute for Biophysical Chemistry, Göttingen
Phone: +49 551 201-1308
E-mail: anne.morbach@mpibpc.mpg.de

Weitere Informationen:

http://www.mpibpc.mpg.de/15377790/pr_1620 – original press release
http://www.mpibpc.mpg.de/cramer – Website of the Department of Molecular Biology at the Max Planck Institute for Biophysical Chemistry, Göttingen
http://www.gagneurlab.in.tum.de – Website of the Computational Biology Group at the Technical University of Munich

Dr. Carmen Rotte | Max-Planck-Institut für biophysikalische Chemie

More articles from Life Sciences:

nachricht Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory

nachricht How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>