Scientists discovered a new way in which microRNAs can determine the fate of cells in the course of their development. This could be a key to understanding how complex organisms are built, say researchers from the Institute of Molecular Pathology (IMP) in Vienna.
A class of genes called microRNAs are known to prevent gene expression. In a recent study, scientists could show that down-regulating genes through microRNAs can determine unique properties of specific cells in an unexpected way. This finding was now reported in the journal “Genes & Development”.
“We set out from a curious observation in the nematode worm C. elegans”, explains Luisa Cochella. “We noticed that the gene mir-791 was expressed specifically in neurons we knew were responsible for the detection of carbon dioxide.” The tiny worm is a common model organism because its anatomy and cell functions are very well-understood and can be observed easily under a microscope. When the worms detect carbon dioxide, they start moving in a characteristic way, giving the scientists an easy tool to indirectly “watch” molecular processes by observing the worms’ behaviour.
When the scientists removed mir-791 from the worms, the animals had trouble responding to carbon dioxide. Normally, mir-791 prevents the expression of two genes exclusively in the neurons that detect carbon dioxide, while these same two genes are expressed in pretty much all other cells of the worm. When this repression mechanism fails, the carbon dioxide sensing neurons do not work properly and animals cannot respond adequately to an environmental cue that, in its natural environment, could determine whether the worm lives or dies. The question how the identity of cells is determined in the course of their development, however, goes far beyond nematodes – it is a fundamental question in biology.
The cells that form our bodies belong to hundreds of different cell types that are shaped by the combinations of genes they express. For example, hemoglobin is produced in red blood cells where it is necessary for oxygen transport, while neurotransmitter receptors are made in neurons where they allow these cells to communicate with each other. On the other hand, a number of genes that are required for more common functions of cells are expressed by most, if not all cells. These are called ubiquitous genes.
In rare cases, ubiquitous genes are not expressed in a specific cell type, where reduced levels of these genes are necessary for the correct function of this particular cell type. The unique properties of a cell can therefore not only be specified by the genes it expresses, but also by the genes it prevents from being expressed. However, the mechanisms that underlie the highly specific repression of genes in some cells have remained puzzling, which is why this study is important.
What was characterised as a developmental mechanism could also be relevant for evolution. The responses of different animals to carbon dioxide vary a lot – some are attracted to it because it is linked to food sources, others flee from it because carbon dioxide is often high in environments with little oxygen. “Given this, our work has the additional implication that microRNAs may be good candidates to give species new tools to adapt to different environments”, says Cochella, who points at the known fact that microRNAs evolve faster than the bigger protein-coding genes.
The IMP, with 15 groups focusing on a diversity of fundamental research questions, provided the perfect place for Cochella and her team to carry out this study. The collaboration with co-author Manuel Zimmer, who has long established ways to quantitatively measure behavioural responses in C. elegans, was instrumental to this project.
In addition, this study provides insight into understanding microRNA functions. “In C. elegans there are between 150 and 200 miRNAs but we only know the functions of 25 or so of them”, says Cochella. This is not only true for the worm, a similarly low fraction of miRNAs has been characterized out of the hundreds found in humans, even though as a whole, miRNAs are essential for animal development and function. This study hints at why miRNA functions have been difficult to uncover: many miRNAs may be only expressed in restricted cell types and finding their functions may require methods as precise as those used in the worm to know exactly which and how cells are affected.
Tanja Drexel, Katharina Mahofsky, Richard Latham, Manuel Zimmer and Luisa Cochella: Neuron-type specific miRNA represses two broadly expressed genes to modulate an avoidance behavior in C. elegans. Genes & Dev., Published in Advance September 29, 2016, doi: 10.1101/gad.287904.116
“Luisa Cochella studies the ways in which genes specify different cell identities, using a tiny worm as a model organism. The microscope image here reveals the cells that allow the worm to smell carbon dioxide.” © IMP/Beck
About Luisa Cochella
Luisa Cochella studies the ways in which gene expression must be regulated during development in order to specify the properties of the hundreds of different cell types that give rise to a complex multicellular animal. She studies this in the tiny nematode worm C. elegans. This worm is an extremely useful model organism to address this question because it is made of relatively few cells that can easily be followed during development.
Luisa Cochella did her undergraduate studies at Universidad de Buenos Aires in her home country Argentina, followed by PhD studies at Johns Hopkins School of Medicine in Baltimore (USA). After her post-doctoral work at Columbia University in New York (USA), Dr. Cochella took the next step in her outstanding academic career and became a group leader at the Research Institute of Molecular Pathology (IMP) in Vienna (Austria), in January 2013.
About the IMP
The renowned Research Institute of Molecular Pathology (IMP) in Vienna is a basic biomedical research institute largely sponsored by Boehringer Ingelheim. With over 200 scientists from 37 nations, the IMP is committed to scientific discovery of fundamental molecular and cellular mechanisms underlying complex biological phenomena. Research areas include cell and molecular biology, neurobiology, disease mechanisms and computational biology.
Dr. Benedikt Mandl
IMP - Communications
Tel. +43 1 79730 3627
Dr. Heidemarie Hurtl | idw - Informationsdienst Wissenschaft
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences