Scientists at the University of Würzburg have chemically modified the enzyme levansucrase using a new method. The enzyme can now produce sugar polymers that are exciting for applications in the food industry and medicine.
Enzymes are tools of nature that accelerate almost all biochemical reactions in living cells as biological catalysts. For this reason, enzymes have been used in the chemical industry for some time now - in detergents and cleaners, toothpastes and shampoos, but also in foods. Enzymes help in the production of paper, textiles, leather, medicines, biofuels and other products.
Enzymes from the tailoring industry
Biochemically, enzymes are proteins that are composed of natural amino acids. They form a three-dimensional structure. Just like a key fits into a lock, each specific molecule fits into an enzyme and the enzyme converts it into a new product.
Technically, it is possible to exchange individual amino acids in an enzyme and thereby change its structure so that it can now process other molecules. In this way, British scientists have created just recently a mutant enzyme that breaks down plastic.
Surface of the levansucrase changed
Chemists from Julius-Maximilians-Universität Würzburg (JMU) in Bavaria, Germany, have now gone a step further in the tailoring of enzymes: "We thought what fascinating possibilities would arise if we could change the surface of enzymes chemically and use the chemical space of molecules," says Jürgen Seibel , Professor of Organic Chemistry at the JMU. "We have developed a reaction that does not occur in nature in this way. It gives us a lot of freedom in reshaping enzyme surfaces. "
As the JMU scientists report in the journal "Chemical Science", they first redesigned the surface of the enzyme levansucrase. Now, the enzyme can convert the table sugar (sucrose) directly into a polymer of fructose building blocks.
"So far, such a synthesis has been possible with levansucrase, but it works much more efficiently with the modified enzyme," explains Seibel. The conversion of the enzyme per second is now significantly higher; moreover, it mainly produces the desired product and no accidental by-products.
Interesting for medicine and food industry
The fructose polymer could be used as a bio-gel for tissue transplantation in medicine or in the food industry – for example as a probiotic supplement in yogurts or baby food. Because like other functional sugars, the polymer could also serve certain intestinal bacteria as food and indirectly exert a health-promoting influence on the intestinal flora of humans.
“Product-Oriented Chemical Surface Modification of a Levansucrase (SacB) via an Ene-type Reaction”, Maria Elena Ortiz-Soto, Julia Ertl, Jürgen Mut, Juliane Adelmann, Thien Anh Le, Junwen Shan, Jörg Teßmar, Andreas Schlosser, Bernd Engels, Jürgen Seibel. Chemical Science 2018 Advance Article, DOI: 10.1039/C8SC01244J
Prof. Dr. Jürgen Seibel, Institute of Organic Chemistry, University of Würzburg, T +49 931 31-85326, email@example.com
Dr. Maria Ortiz-Soto, Institute of Organic Chemistry, University of Würzburg, T +49 931 3- 88733, firstname.lastname@example.org
Robert Emmerich | Julius-Maximilians-Universität Würzburg
Tag it EASI – a new method for accurate protein analysis
19.06.2018 | Max-Planck-Institut für Biochemie
How to track and trace a protein: Nanosensors monitor intracellular deliveries
19.06.2018 | Universität Basel
Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...
Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.
Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...
The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.
Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.
An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.
Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...
Light detection and control lies at the heart of many modern device applications, such as smartphone cameras. Using graphene as a light-sensitive material for...
13.06.2018 | Event News
08.06.2018 | Event News
05.06.2018 | Event News
19.06.2018 | Physics and Astronomy
19.06.2018 | Life Sciences
19.06.2018 | Physics and Astronomy