This technique uses Optical Projection Tomography, which is “similar to X-rays, but uses light,” explains UC3M researcher Jorge Ripoll, from the UC3M Department of Bioengineering and Aerospace Engineering. With this technique, it is possible to use optical markers which are often used with transgenic animals. One such marker is green fluorescent protein. Thanks to this substance, one can observe the anatomy and functions of living organisms like flies or very small fish.
This research, recently published in the journal Scientific Reports, makes it possible to follow the development of living organisms up to three millimetres long with three-dimensional images. These organisms, such as the zebrafish or the fruit fly, are frequently used in microscopic research. The fruit fly (Drosophila melanogaster), for example, has a genetic code where the counterparts of more than 60% of the genes of human illnesses can be found.
Ripoll says that the advance consists of being able to follow the development of these organisms, which normally appear opaque when viewed with a conventional microscope because they diffuse a lot of light when they approach adulthood. “It helps us visualize new stages,” says Ripoll. In this he way, he says, although “this technique cannot be used on living humans because our tissue is very opaque, it can be used to “take three-dimensional measurements of biopsies, which is very valuable to a surgeon,” as it would permit her to know if the surgery went as desired.
The way to put this technique into practice is simple, says Ripoll. “It consists of a source of light that stimulates the fluorescence and a camera that detects it” and has only one requirement: “that the sample rotates” as if X-rays were being taken of it. Afterwards, with that information, “we must construct a three-dimensional image,” he explains.
The development of this technique has been possible thanks to the support, among others, of researchers from the Chinese Academy of Sciences. These researchers “were responsible for creating the software so that the obtaining of images could be fast and effective,” he says. Along with this, he comments that the technology on which the techniques his Chinese colleagues use are based has its origins in the development of video games.
Participating in this research are Alicia Arranz, from the Swiss Federal Institute of Technology; Di Dong, from the Chinese Academy of Sciences; Shouping Zhu and Jie Tian, from the School of Life Sciences and Technology; Charalambos Savakis, from the BSRC Alexander Fleming and Jorge Ripoll, from the UC3M Department of Bioengineering and Aerospace Engineering.
Subtítulos en castellano, inglés y chinoEnglish, chinese and spanish subtitles
Javier Alonso Flores | AlphaGalileo
Matchmaking with consequences
17.10.2017 | Julius-Maximilians-Universität Würzburg
Taking screening methods to the next level
17.10.2017 | IMBA - Institut für Molekulare Biotechnologie der Österreichischen Akademie der Wissenschaften GmbH
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
It's possible to produce hydrogen to power fuel cells by extracting the gas from seawater, but the electricity required to do it makes the process costly. UCF...
Mercury, our smallest planetary neighbor, has very little to call an atmosphere, but it does have a strange weather pattern: morning micro-meteor showers.
Recent modeling along with previously published results from NASA's MESSENGER spacecraft -- short for Mercury Surface, Space Environment, Geochemistry and...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
17.10.2017 | Life Sciences
17.10.2017 | Event News
17.10.2017 | Physics and Astronomy