Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New procedure to obtain induced pluripotent stem cells

10.06.2015

A new protocol that simplifies the process that allows induced pluripotent stem cells was developed at the Center for Biomedicine of the European Academy of Bolzano. While the traditional methodology requires fresh blood, the new procedure allows cells from frozen blood samples to regress to a similar state to that of embryonic stem cells. The reprogrammed cells can be used to understand how some diseases develop and to test new therapies. The new protocol reduces costs and work time in the laboratory.

For their ability to differentiate into other cell types, the embryonic stem cells hold a large potential in the medical industry. Their use, however, poses ethical questions due to the fact that in order to obtain them, it is necessary to destroy the embryo. For this reason, medical researches use the so-called induced pluripotent stem cells (iPSC).


Research on iPS-cells at the EURAC laboratory

EURAC/Bortolotti

In fact, it is possible to reprogram adult cells that can be obtained by simply drawing blood and making them “regress” to a similar state to that of embryonic stem cells. The reprogrammed cells (iPSC) are capable of creating all the cell types of an adult organism, such as brain or heart cells.

The iPSC technology is revolutionizing medical science, allowing for the exploration of molecular mechanisms that regulate diseases, providing new therapeutic targets, and providing opportunities for the discovery of new drugs.

Thanks to the iPSC, the possibility to develop a truly personalized medicine will become more realistic, enabling the testing of specific drugs on cells, such as neurons and cardiomyocytes, otherwise impossible to isolate from patients with specific diseases.

“The procedure that we have developed simplifies the process that enables these cells to be obtained. With the traditional method, the blood is centrifuged with different reagents, capable of separating the blood cells according to their size. Our protocol, on the other hand, does not require the use of reagents.

This allows for the reduction of costs, time, and the complexity of procedures to be performed in the laboratory”, explain Viviana Meraviglia and Alessandra Zanon, researchers at EURAC Center for Biomedicine and main authors of the study.

“The big advantage of our method is that it can also be applied to blood samples previously collected and preserved in a biobank. We will be able to access samples collected in other studies that we have performed or from biobanks in other research centers”, continues Alessandra Rossini, study coordinator.
The research performed by the EURAC Center for Biomedicine focuses especially on cardiovascular and neurological diseases, such as Parkinson´s.

Currently, researchers are engaged in the differentiation of the cardiomyocyte induced pluripotent cells to study a genetic disease associated with a right ventricular arrhythmia (arrhythmogenic right ventricular dysplasia, ARVD) and in dopaminergic neurons, meaning specific brain cells, to study the development of Parkinson´s.

The study was published in the international scientific journal ”JoVE”, Journal of Visualized Experiments (http://www.jove.com/video/52885), who invited a troupe into the Center for Biomedicine laboratories to film all the steps of the new methodology in detail, so they could be replicated in other research centers.

Laura Defranceschi | idw - Informationsdienst Wissenschaft
Further information:
http://www.eurac.edu

More articles from Life Sciences:

nachricht Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo

nachricht Full of hot air and proud of it
18.04.2018 | University of Pittsburgh

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

Im Focus: The Future of Ultrafast Solid-State Physics

In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.

Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Diamond-like carbon is formed differently to what was believed -- machine learning enables development of new model

19.04.2018 | Materials Sciences

Electromagnetic wizardry: Wireless power transfer enhanced by backward signal

19.04.2018 | Physics and Astronomy

Ultrafast electron oscillation and dephasing monitored by attosecond light source

19.04.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>