Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New procedure to obtain induced pluripotent stem cells

10.06.2015

A new protocol that simplifies the process that allows induced pluripotent stem cells was developed at the Center for Biomedicine of the European Academy of Bolzano. While the traditional methodology requires fresh blood, the new procedure allows cells from frozen blood samples to regress to a similar state to that of embryonic stem cells. The reprogrammed cells can be used to understand how some diseases develop and to test new therapies. The new protocol reduces costs and work time in the laboratory.

For their ability to differentiate into other cell types, the embryonic stem cells hold a large potential in the medical industry. Their use, however, poses ethical questions due to the fact that in order to obtain them, it is necessary to destroy the embryo. For this reason, medical researches use the so-called induced pluripotent stem cells (iPSC).


Research on iPS-cells at the EURAC laboratory

EURAC/Bortolotti

In fact, it is possible to reprogram adult cells that can be obtained by simply drawing blood and making them “regress” to a similar state to that of embryonic stem cells. The reprogrammed cells (iPSC) are capable of creating all the cell types of an adult organism, such as brain or heart cells.

The iPSC technology is revolutionizing medical science, allowing for the exploration of molecular mechanisms that regulate diseases, providing new therapeutic targets, and providing opportunities for the discovery of new drugs.

Thanks to the iPSC, the possibility to develop a truly personalized medicine will become more realistic, enabling the testing of specific drugs on cells, such as neurons and cardiomyocytes, otherwise impossible to isolate from patients with specific diseases.

“The procedure that we have developed simplifies the process that enables these cells to be obtained. With the traditional method, the blood is centrifuged with different reagents, capable of separating the blood cells according to their size. Our protocol, on the other hand, does not require the use of reagents.

This allows for the reduction of costs, time, and the complexity of procedures to be performed in the laboratory”, explain Viviana Meraviglia and Alessandra Zanon, researchers at EURAC Center for Biomedicine and main authors of the study.

“The big advantage of our method is that it can also be applied to blood samples previously collected and preserved in a biobank. We will be able to access samples collected in other studies that we have performed or from biobanks in other research centers”, continues Alessandra Rossini, study coordinator.
The research performed by the EURAC Center for Biomedicine focuses especially on cardiovascular and neurological diseases, such as Parkinson´s.

Currently, researchers are engaged in the differentiation of the cardiomyocyte induced pluripotent cells to study a genetic disease associated with a right ventricular arrhythmia (arrhythmogenic right ventricular dysplasia, ARVD) and in dopaminergic neurons, meaning specific brain cells, to study the development of Parkinson´s.

The study was published in the international scientific journal ”JoVE”, Journal of Visualized Experiments (http://www.jove.com/video/52885), who invited a troupe into the Center for Biomedicine laboratories to film all the steps of the new methodology in detail, so they could be replicated in other research centers.

Laura Defranceschi | idw - Informationsdienst Wissenschaft
Further information:
http://www.eurac.edu

More articles from Life Sciences:

nachricht Hunting pathogens at full force
22.03.2017 | Helmholtz-Zentrum für Infektionsforschung

nachricht A 155 carat diamond with 92 mm diameter
22.03.2017 | Universität Augsburg

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Pulverizing electronic waste is green, clean -- and cold

22.03.2017 | Materials Sciences

Astronomers hazard a ride in a 'drifting carousel' to understand pulsating stars

22.03.2017 | Physics and Astronomy

New gel-like coating beefs up the performance of lithium-sulfur batteries

22.03.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>