Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New nanoscopic tools to study ligand-binding of receptors

19.11.2015

A new high resolution method developed by an international team of scientists including Robert Tampé and Ralph Wieneke from Goethe University Frankfurt now allows for the first time precise identification and quantification of interactions of a receptor with two ligands simultaneously. The new method has been published in the latest edition of the journal Nature Communications.

Signalling processes in organisms are governed by specific extracellular and intracellular interactions and involve hundreds of different functionally highly versatile receptors situated in cell membranes.


A new high resolution method allows for the first time precise identification and quantification of interactions of a receptor with two ligands simultaneously.

GU

For scientists wishing to understand signalling processes the situation is made more complex by the receptors not only being unevenly distributed and often able to bind more than one ligand but also by the same type of receptor being able to bind a ligand strongly, weakly or not at all. New methods that allow precise quantifications of such complex interactions are urgently required.

A new high resolution method developed by an international team of scientists including Robert Tampé and Ralph Wieneke from Goethe University Frankfurt now allows for the first time precise identification and quantification of interactions of a receptor with two ligands simultaneously. The new method has been published in the latest edition of the journal Nature Communications.

Atomic force microscopy (AFM) is a powerful technique for nanoscale characterization of surfaces. It makes use of a cantilever with an extremely fine tip. Force-distance curve-based atomic force microscopy (FD-based AFM) combines high-resolution imaging and single-molecule force spectroscopy.

In studies using biological samples, the AFM tip approaches and retracts from the sample for each pixel. FD-based AFM methods use different coatings of the AFM tip as a toolbox and such methods have made impressive progress in recent years.

For the detection of specific binding sites FD-based AFM requires tethering of a ligand to the AFM tip. While contouring protein complexes in a membrane such functionalized AFM tips can then measure the interactions of the tethered ligand to the protein. It had not been possible to image single membrane receptors and simultaneously detect their interactions with more than one ligand, but the new method has overcome this hurdle.

For their proof of principle the scientists used the human protease-activated receptor 1 (PAR1), one of the large family of G-protein-coupled membrane receptors. GPCRs mediate most cellular responses to hormones and neurotransmitters, as well as being responsible for vision, olfaction and taste.

GPCRs can coexist in different functional states in the cell membrane and can bind various ligands at different strength or affinity. The GPCR PAR1 is activated by the coagulation protease thrombin which triggers signalling cascades to initiate cellular responses that help orchestrate haemostasis, thrombosis, inflammation and possibly also tissue repair.

With the aid of their new FD-based AFM method human PAR1 in proteoliposomes could be imaged while simultaneously detecting extracellular and intracellular interactions of PAR1 with two ligands. The surface chemistry and nanoscopic method developed are applicable to a range of biological systems in vitro and in vivo.

Contact:
Robert Tampé, Institute of Biochemistry, tampe@em.uni-frankfurt.de
Goethe University Frankfurt, Max von Laue Straße 9, 60438 Frankfurt/Germany

Dr. Anne Hardy | idw - Informationsdienst Wissenschaft
Further information:
http://www.uni-frankfurt.de

More articles from Life Sciences:

nachricht Bolstering fat cells offers potential new leukemia treatment
17.10.2017 | McMaster University

nachricht Ocean atmosphere rife with microbes
17.10.2017 | King Abdullah University of Science & Technology (KAUST)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Ocean atmosphere rife with microbes

17.10.2017 | Life Sciences

Neutrons observe vitamin B6-dependent enzyme activity useful for drug development

17.10.2017 | Life Sciences

NASA finds newly formed tropical storm lan over open waters

17.10.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>