Researchers at the Cells-in-Motion Cluster of Excellence have developed a new method for producing digital 3D reconstructions of blood and lymphatic vessels from tissue samples and then creating images of them for analysis. The study has been published in the “JCI Insight” journal.
When researchers and physicians analyse tissue, for example in order to investigate any pathological changes, they often look at the tissue samples under the light microscope. However, producing meaningful images is not always easy.
Researchers at the Cells-in-Motion Cluster of Excellence at the University of Münster and at the Max Planck Institute for Molecular Biomedicine in Münster have now developed a new method which, in the case of lymphoedema, can create digital 3D images of blood vessels and lymphatic vessels of entire tissue biopsies.
This method will help to analyse the underlying changes of the blood and lymphatic vessels in lymphoedema in a more detailed way. “We’re doing a digital three-dimensional histopathology,” explains Dr. René Hägerling, lead author of the study which has just been published in the latest issue of the “JCI Insight” journal.
The process has involved interdisciplinary collaboration between biochemists, chemists, computer scientists, biologists and physicians. The researchers analysed three skin biopsies taken from healthy persons and one skin biopsy of a patient with lymphoedema.
Using light sheet microscopy they produced thousands of individual optical sections for each sample. Using a special programming system called Voreen the researchers assembled the individual optical sections on the computer and produced a three-dimensional reconstruction of the tissue structure.
The new method – called VIPAR – enables researchers for the first time to generate 3D reconstructions of skin biopsies, visualize them and extract characteristic parameters of the tissue. This method differs from traditional histological analyses, in which a tissue sample is sliced into many sections and each individual section is observed two-dimensionally.
Hägerling R, Drees D, Scherzinger A, Dierkes C, Martin-Almedina S, Butz S, Gordon K, Schäfers M, Hinrichs K, Ostergaard P, Vestweber D, Goerge T, Mansour S, Jiang X, Mortimer PS, Kiefer F. VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations. JCI Insight 2017;2, DOI 10.1172/jci.insight.93424.
https://www.uni-muenster.de/Cells-in-Motion/newsviews/2017/08-28.html - The detailed story on the Website of the Cells-in-Motion Cluster of Excellence
https://insight.jci.org/articles/view/93424 - Abstract (published in the “JCI Insight” journal)
Juliane Albrecht | idw - Informationsdienst Wissenschaft
22.02.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau
Separate brain systems cooperate during learning, study finds
22.02.2018 | Brown University
Quantum computers may one day solve algorithmic problems which even the biggest supercomputers today can’t manage. But how do you test a quantum computer to...
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...
Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.
But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...
Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.
The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...
15.02.2018 | Event News
13.02.2018 | Event News
12.02.2018 | Event News
22.02.2018 | Life Sciences
22.02.2018 | Physics and Astronomy
22.02.2018 | Earth Sciences