Scientists at the Leibniz-Institute of Plant Biochemistry (IPB) in Halle/Saale (Germany) have fully elucidated the biosynthesis of carnosic acid. This discovery allowed the plant researchers around Prof. Alain Tissier to produce the economically valuable plant material by biotechnological means in yeast cells. The project was published in the renowned journal Nature Communications.
Carnosic acid is a natural antioxidant that is found in the leaves of rosemary and sage. It is used worldwide as a preservative and flavor in meat products, oils, fats, sauces and animal feed. Carnosic acid, for which the demand is steadily increasing, is still extracted from rosemary plants, which grow slowly.
Carnosic acid is still obtained from rosemary. However, biotechnological production processes could be developed soon.
Dried leaves of sage or rosemary contain at most 2.5 percent of carnosic acid, necessitating a large amount of plant material to ensure the production of the antioxidant on industrial scale. Furthermore, the complex structure of carnosic acid makes an industrial synthetic process unrealistic.
The biosynthesis of carnosic acid within the plant takes place in several reaction steps, which are catalyzed by different enzymes. The enzyme that catalyzes the last step of the reaction chain had not yet been discovered. This knowledge-gap has now been closed by the IPB researchers.
They discovered an additional, previously unknown intermediate and also new enzymes, which were described and characterized by them. With the knowledge of all involved reaction partners, the scientists were able to introduce the genes coding for the corresponding enzymes into yeast cells and make them produce carnosic acid. This is the first step in the development of a biotechnological production process for the antioxidant.
Carnosic acid is also the starting material for the biosynthesis of many other phenolic diterpenes, which act as bioactive substances against inflammation, cancer and various neurodegenerative diseases.
Also for this reason, it will be interesting to produce carnosic acid in the future with biotechnology-based processes and thus independently of climate fluctuations, soil quality and harvest yields.
Ulschan Scheler, Wolfgang Brandt, Andrea Porzel, Kathleen Rothe, David Manzano, Dragana Bozic, Dimitra Papaefthimiou, Gerd Ulrich Balcke, Anja Henning, Swanhild Lohse, Sylvestre Marillonnet, Angelos K. Kanellis, Albert Ferrer & Alain Tissier. Elucidationof the bioynthesis of carnosic acid and its reconstitution in yeast. Nature Communications 7: 12942, doi:10.1038/ncomms12
Prof. Alain Tissier
Leibniz Institute of Plant Biochemistry
Tel.: +49 345 5582 1500
Dipl.Biol. Sylvia Pieplow | idw - Informationsdienst Wissenschaft
New risk factors for anxiety disorders
24.02.2017 | Julius-Maximilians-Universität Würzburg
Stingless bees have their nests protected by soldiers
24.02.2017 | Johannes Gutenberg-Universität Mainz
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
24.02.2017 | Life Sciences
24.02.2017 | Life Sciences
24.02.2017 | Trade Fair News