Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Nanotechnology reveals hidden depths of bacterial 'machines'

09.06.2017

New research from the University of Liverpool, published in the journal Nanoscale, has probed the structure and material properties of protein machines in bacteria, which have the capacity to convert carbon dioxide into sugar through photosynthesis.

Cyanobacteria are a phylum of bacteria that produce oxygen and energy during photosynthesis, similar to green plants. They are among the most abundant organisms in oceans and fresh water. Unique internal 'machines' in cyanobacteria, called carboxysomes, allow the organisms to convert carbon dioxide to sugar and provide impacts on global biomass production and our environment.


This is an illustration of a carboxysome.

Credit

Dr Luning Liu, University of Liverpool

Carboxysomes are nanoscale polyhedral structures that are made of several types of proteins and enzymes. So far, little is known about how these 'machines' are constructed and maintain their organisation to perform carbon fixation activity.

Researchers from the University's Institute of Integrative Biology, led by Royal Society University Research Fellow Dr Luning Liu, examined in depth the native structure and mechanical stiffness of carboxysomes using advanced microscopes and biochemical approaches.

For the first time, the researchers were able to biochemically purify active carboxysomes from cyanobacteria and characterize their carbon fixation activity and protein composition. They then used electron microscopy and atomic force microscopy to visualise the morphology and internal protein organization of these bacterial machines.

Furthermore, the intrinsic mechanical properties of the three-dimensional structures were determined for the first time. Though structurally resembling polyhedral viruses, carboxysomes were revealed to be much softer and structurally flexible, which is correlated to their formation dynamics and regulation in bacteria.

Dr Liu, said: "It's exciting that we can make the first 'contact' with these nano-structures and understand how they are self-organised and shaped using state-of-the-art techniques available at the University. Our findings provide new clues about the relationship between the structure and functionality of native carboxysomes."

The self-assembly and modularity features of carboxysomes make them interesting systems for nanoscientists, synthetic biologists and bioengineers, who hope to find ways to design new nanomaterials and nano-bioreactors.

"We're now just starting to understand how these bacterial machines are built and work in nature. Our long-term vision is to harness the knowledge to make further steps towards better design and engineering of bio-inspired machines," added Dr Liu, "The knowledge and techniques can be extended to other biological machines."

###

The project was done in collaboration with Professor Rob Beynon at the University's Centre for Proteome Research and the Centre for Cell Imaging and funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and a Royal Society University Research Fellowship.

The paper 'Direct characterization of the native structure and mechanics of cyanobacterial carboxysomes' is published in the journal Nanoscale [DOI: 10.1039/C7NR02524F]

Nicola Frost | EurekAlert!

More articles from Life Sciences:

nachricht More genes are active in high-performance maize
19.01.2018 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht How plants see light
19.01.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Artificial agent designs quantum experiments

On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.

We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...

Im Focus: Scientists decipher key principle behind reaction of metalloenzymes

So-called pre-distorted states accelerate photochemical reactions too

What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...

Im Focus: The first precise measurement of a single molecule's effective charge

For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.

Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...

Im Focus: Paradigm shift in Paris: Encouraging an holistic view of laser machining

At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.

No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...

Im Focus: Room-temperature multiferroic thin films and their properties

Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.

Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

10th International Symposium: “Advanced Battery Power – Kraftwerk Batterie” Münster, 10-11 April 2018

08.01.2018 | Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

 
Latest News

Let the good tubes roll

19.01.2018 | Materials Sciences

How cancer metastasis happens: Researchers reveal a key mechanism

19.01.2018 | Health and Medicine

Meteoritic stardust unlocks timing of supernova dust formation

19.01.2018 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>