Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Molecular volume control

22.08.2017

About two years ago, scientists from the University of Würzburg discovered that a certain class of receptors is capable of perceiving mechanical stimuli. Now they have begun to unravel the molecular mechanisms behind the discovery.

The receptor studied by scientists from the universities of Würzburg and Leipzig over the past years works similarly to the volume control of a stereo which enhances or attenuates the incoming signal. The receptor in question is called latrophilin/CIRL.


The larval Drosophila chordotonal organ seen under the scanning electron microscope. This sensory functional unit modulates the processing of mechanical stimuli by means of the latrophilin receptor.

(Photo: Scholz et al., 2017)

A little more than two years ago, the researchers had surprised the scientific community by proving that certain receptors, including latrophilin, respond to mechanical stimuli from the environment for example vibration, sound waves or expansion. By doing so, the receptors help organisms to hear, perceive movements and control their own movements.

How the information gets inside the cell

At the time, however, the details of the receptors' contribution were still unclear, i.e. how the process works at the molecular level. In the meantime, the researchers have been able to shed light on some crucial details. They present their results in the current issue of the scientific journal eLife. The lead authors of the study are Dr Robert Kittel, who heads a working group at the Institute of Physiology/Department of Neurophysiology at the University of Würzburg, and Professor Tobias Langenhan, who recently relocated from Würzburg to the University of Leipzig.

"In order for cells to perceive and respond to external stimuli, the information must somehow get inside the cell," Robert Kittel explains the central aspect of the study. This may be accomplished through ion channels where a mechanical stimulus is converted into an electrical response in a very straightforward and fast process.

With the latrophilin receptor things are different: "It does not form a channel and it does not forward the stimulus electrically," Kittel says. Instead, it activates intracellular messengers that trigger special signal cascades inside the cell which ultimately also affect the ion channels. According to Kittel, the receptor thus has a modulating effect on stimulus perception like some kind of volume controller.

Collaboration with numerous experts

The study just published is the result of collaborating with specialists from various domains at the University of Würzburg – an aspect which Robert Kittel particularly appreciates.

One of the contributing experts is the plant physiologist Professor Georg Nagel who was one of the scientists who discovered a celebrated technique which became known as "optogenetics". The underlying principle: Nagel characterizes ion channels and enzymes that can be controlled with light. Robert Kittel and Tobias Langenhan used the larvae of Drosophila, the fruit fly, for their experiments which are almost transparent so that the researchers were able to study the functioning of the receptors with simple flashes of light.

The second expert involved was Professor Markus Sauer, head of the Department of Biotechnology and Biophysics at University of Würzburg's Biocenter. With his team, Sauer developed special forms of high-resolution fluorescence microscopy. This "super resolution" microscopy allows imaging cellular structures and molecules with up to tenfold increased resolution compared to conventional optical microscopes. "By using super-resolution microscopy, we were able to pinpoint the position of the cell membrane where the receptor is located," Robert Kittel says.

Dr. Isabella Maiellaro and Professor Esther Asan are also specialists in the field of imaging procedures. By teaming up with Isabella Maiellaro from the Department of Pharmacology, the researchers were able to directly visualize the intracellular receptor signal. Esther Asan, Professor at the Institute of Anatomy and Cell Biology II at the University of Würzburg, also contributed to the success of the study with her expertise in electron microscopy.

Moreover, the project was supported by the extensive experience of Professor Matthias Pawlak at the Institute of Physiology of the University of Würzburg in the field of sensory physiology and Dr Simone Prömel, a pharmacologist at the University of Leipzig. Robert Kittel sees these collaborations as a good example of how modern biotechnological methods can help answer physiological questions.

A very important molecular family

Latrophilin/CIRL is a member of a family of molecules that has more than 30 members in humans: the so-called adhesion GPCRs, a subgroup of the G protein-coupled receptors (GPCRs). Hundreds of them are encoded in the human genome; their importance is underpinned among others by the fact that around half of all prescription drugs target these receptors and help treat common diseases such as high blood pressure, asthma or Parkinson's.

This shows just how important the research results of the scientists from Würzburg and Leipzig are. After all, knowing what is going on inside the cells is a prerequisite for developing a better understanding of pathological processes and designing new therapies. "The cell biology processes are well conserved in terms of evolution," Robert Kittel says. Similar mechanisms are also at work in human cells.

Robert Kittel and Tobias Langenhan are also members of a research unit funded by Deutsche Forschungsgemeinschaft (DFG FOR 2149) which studies the signalling behaviour of adhesion GPCRs. The current study harnesses the good experimental accessibility of Drosophila to bring new technologies into a biomedical context more quickly. This allows basic molecular mechanisms to be described for the first time. These mechanisms are now to be studied in further organisms and physiological contexts in collaboration with other scientists.

Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons. Nicole Scholz, Chonglin Guan, Matthias Nieberler, Alexander Grotemeyer, Isabella Maiellaro, Shiqiang Gao, Sebastian Beck, Matthias Pawlak, Markus Sauer, Esther Asan, Sven Rothemund, Jana Winkler, Simone Prömel, Georg Nagel, Tobias Langenhan, Robert J Kittel. eLife 2017;6:e28360. DOI: 10.7554/eLife.28360

Contact

Dr Robert J. Kittel, T: +49 931 31-86046; robert.Kittel@uni-wuerzburg.de

Prof. Tobias Langenhan, T: +49 341 97-22100; tobias.langenhan@uni-leipzig.de

Robert Emmerich | Julius-Maximilians-Universität Würzburg
Further information:
http://www.uni-wuerzburg.de

More articles from Life Sciences:

nachricht One step closer to reality
20.04.2018 | Max-Planck-Institut für Entwicklungsbiologie

nachricht The dark side of cichlid fish: from cannibal to caregiver
20.04.2018 | Veterinärmedizinische Universität Wien

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Spider silk key to new bone-fixing composite

University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.

Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.

Im Focus: Writing and deleting magnets with lasers

Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.

Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...

Im Focus: Gamma-ray flashes from plasma filaments

Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.

The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...

Im Focus: Basel researchers succeed in cultivating cartilage from stem cells

Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.

Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...

Im Focus: Like a wedge in a hinge

Researchers lay groundwork to tailor drugs for new targets in cancer therapy

In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

IWOLIA: A conference bringing together German Industrie 4.0 and French Industrie du Futur

09.04.2018 | Event News

 
Latest News

Magnetic nano-imaging on a table top

20.04.2018 | Physics and Astronomy

Start of work for the world's largest electric truck

20.04.2018 | Interdisciplinary Research

Atoms may hum a tune from grand cosmic symphony

20.04.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>