Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Is a small artificially composed virus fragment the key to a Chikungunya vaccine?

24.04.2015

The mosquito transmitted Chikungunya virus, which causes Chikungunya fever, is spreading continuously. No vaccine is so far available. Researchers of the Paul-Ehrlich-Institut have experimentally recombined segments of the virus surface protein E2, thus creating artificial proteins. The domain generated that way – "sAB+" – was able to confer a protective effect against Chikungunya virus to the animal. An immunization by means of this small protein fragment could thus provide a suitable approach to developing a Chikungunya vaccine. PLoS Neglected Tropical Diseases reports on the research results in its online edition of 23 April 2015 in the evening.

The Chikungunya virus (CHIKV) is transmitted by Aedes mosquitoes and causes an infection in humans known as Chikungunya fever. CHIKV occurs in the tropical and subtropical parts of the world. Regions where it has already caused epidemics include Africa, territories around the Indian Ocean, Southeast Asia, and meanwhile also the Caribbean, Central America, and South America.


Three dimensional structure of the Chikungunya virus envelope protein E2. Areas marked in red were used for the approaches to a vaccine.

www.rcsb.org/pdb/explore/explore.do?structureId=3N44

Around 1.2 million people are estimated to be infected so far during an epidemic in America. Since the Aedes albopictus mosquito, also known as Asian tiger mosquito, has now reached southern Europe and the USA, we are faced with further spreading of the virus. The Paul-Ehrlich-Institut has issued the regulation in 2007 that after returning from an endemic area, blood donors must be deferred from donating blood for at least two weeks to prevent an infection via the blood stream.

The disease is characterized by fever and severe joint pain, hence its name, which means "that which bends up". In 30 to 40 percent of the cases, these joint pains can last several months or even up to several years.

Attempts at developing suitable vaccines have up to now been unsuccessful. To develop an effective vaccine, it is imperative to identify a suitable antigen structure of the virus which will create an effective immune response in humans. Previous approaches have used the entire E2 surface protein as a basis for the vaccine, partly in combination with other virus proteins. These proteins, however, have a relatively large structure, which would make commercial vaccine production difficult.

Professor Barbara Schnierle, head of the section "AIDS, New and Emerging Pathogens" of the division Virology at the Paul-Ehrlich-Institut and her team have investigated whether smaller more specific and less complex-to-be produced parts of E2 would suffice for conferring a protective immune response. Based on the three-dimensional structure of the protein, the researchers of the PEI selected different areas exposed on the surface to join them together, thus creating several artificial protein fragments.

After production in E. coli and purification, mice were immunized with these protein fragments, and their blood was examined for neutralizing antibodies later on. In this experiment, one fragment, described as sAB+, proved to be the most effective one to induce neutralizing antibodies. It was used to immunize mice which were then infected by the wild-type Chikungunya virus.

Compared with non-vaccinated animals, the mice treated showed significantly less virus RNA in the blood – a sign of partial immune protection. ""Our research work shows that single and artificially composed fragments of the Chikungunya virus surface protein may suffice to induce a partially protective immune response. We consider our vaccine approach as promising for further development"", said Professor Schnierle in her explanation of the research results.

Original publication
Weber C, Büchner SM, Schnierle BS (2015):
A Small Antigenic Determinant of the Chikungunya Virus E2 Protein Is Sufficient to Induce Neutralizing Antibodies which Are Partially Protective in Mice.
PLoS Negl Trop Dis 9: e0003684.
DOI:10.1371/journal.pntd.0003684

The Paul-Ehrlich-Institut, the Federal Institute for Vaccines and Biomedicines, in Langen near Frankfurt/Main is a senior federal authority reporting to the Federal Ministry of Health (Bundesministerium für Gesundheit, BMG). It is responsible for the research, assessment, and marketing authorisation of biomedicines for human use and immunological veterinary medicinal products. Its remit also includes the authorisation of clinical trials and pharmacovigilance, i.e. recording and evaluation of potential adverse effects.

Other duties of the institute include official batch control, scientific advice and inspections. In-house experimental research in the field of biomedicines and life science form an indispensable basis for the manifold tasks performed at the institute.

The Paul-Ehrlich-Institut, with its roughly 800 members of staff, also has advisory functions nationally (federal government, federal states (Länder)), and internationally (World Health Organisation, European Medicines Agency, European Commission, Council of Europe etc.).

Weitere Informationen:

http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003684 - Online Version of the Publication

Dr. Susanne Stöcker | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory

nachricht How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>