Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Introduction of a novel system for in vitro analyses of zebrafish oligodendrocyte progenitor cells

23.10.2017

Introduction of a novel, easy-to-use and highly reproducible culture system for in vitro analyses of zebrafish oligodendrocyte progenitor cells (OPCs)

Dr. Michell M. Reimer, group leader at the Center for Regenerative Therapies Dresden (CRTD), Cluster of Excellence at the TU Dresden, and his team introduce a novel, easy-to-use, and highly reproducible OPC culture platform for adult zebrafish cells.


Dr. Michell M. Reimer

© CRTD


Main steps to obtain a highly pure adult zebrafish spinal oligodendrocyte progenitor cell (OPC) population

2017 Kroehne, Tsata, Marrone, Froeb, Reinhardt, Gompf, Dahl, Sterneckert and Reimer

This system will help to unravel the molecular and cellular programs that enable zebrafish to functionally regenerate spinal cord injuries. The results of this study have been published in the scientific journal Frontiers in Cellular Neuroscience.

Spinal cord injuries result from a blunt or penetrating trauma. This is generally caused by accidents that occur during sport activities or when driving. Injuries of the spinal cord can lead to extreme pain (e.g. pressure in the head, neck or back), the loss of sensation (e.g. in fingers or feet), the loss of control over different parts of the body, an abnormal sense of balance and many other symptoms.

According to the World Health Organization (WHO)*, as many as 500,000 people suffer from spinal cord injuries each year*. Humans do not regain spinal cord function after injury. However, zebrafish have the remarkable ability to functionally recover from spinal cord injury. They repair injured connections, replace damaged motor neurons and oligodendrocytes, enabling them to regain full movement within six weeks after injury.

The study introduced here focused on a population of support cells in the spinal cord that helps to protect surviving nerve cells (neurons) after injury: oligodendrocytes and their precursor cells. Oligodendrocytes, the cells that are known to produce the myelin sheaths which enable saltatory conduction of action potentials along the myelinated axons, are modulators of signal transmission along neuronal connections (axons) and also promote neuronal survival by providing metabolic support.

Oligodendrocyte death, occurring after a spinal cord injury, activates a process called de-myelination that results first in damage to surviving neuronal connections and finally in death of the affected neurons. Although lost mature oligodendrocytes can principally be replaced by resident oligodendrocyte progenitor cells (OPCs) this does not happen sufficiently enough in the human spinal cord after injury. Improving recruitment, activation and differentiation of OPCs is therefore hypothesised to improve functional outcome after a spinal cord injury in humans.

Here Dr. Reimer and his team asked the question, ‘what happens to mature oligodendrocytes after a spinal cord injury in adult zebrafish?’. They found that, like in humans, oligodendrocytes near a spinal cord injury site are massively lost within a week. However, two weeks after injury they found that the oligodendrocyte population was largely re-established, showing the remarkable regenerative capacity of the adult zebrafish spinal cord.

These results placed the resident OPC population in the focus of interest: what are the signals that control and enable the activation of these precursor cells in the adult zebrafish spinal cord? Dr. Reimer and his team decided to establish a novel in vitro platform to analyse zebrafish OPCs independently of the body, as this enables better control over the cells and opens up the possibility for novel methods of analysis. They developed a streamlined and fast, though inexpensive, method that allows direct access to a pure and vital population of zebrafish OPCs in less than 2 hours.

This simple protocol is based on automated fluorescent activated cell sorting (FACS) of OPCs. Using novel culture conditions Dr. Reimer’s team has shown it is now possible to maintain the cells for 16 days in vitro. Finally, they demonstrated that zebrafish OPCs differentiate into mature oligodendrocytes when cultured together with human motor neurons, differentiated from induced pluripotent stem cells. This shows that the basic mechanisms of oligodendrocyte differentiation are conserved across species and that understanding the regulation of zebrafish OPCs can contribute to the development of new treatment for human diseases.

As a next step, Dr. Reimer’s research team intend to analyse the effect of different drugs on zebrafish OPCs in order to potentially identify a method to improve functional spinal cord repair in humans.

Before becoming a research group leader at the CRTD in 2014 (for Regulation of developmental and regenerative processes in the spinal cord), the biologist Michell Reimer worked as a Post-Doctoral Fellow at the Centre for Neuroregeneration and the Centre for Cognitive and Neural Systems at the University of Edinburgh (UK) since 2009. From 2005-2008, Michell Reimer completed his PhD in the field of neuroscience at the Centre for Neuroscience Research, University of Edinburgh.

*http://www.who.int/mediacentre/news/releases/2013/spinal-cord-injury-20131202/en...

Publication
Title: Primary Spinal OPC Culture System from Adult Zebrafish to Study Oligodendrocyte Differentiation In Vitro
Kroehne V., Tsata V., Marrone L., Fröb C., Reinhardt S., Gompf A., Dahl A., Sterneckert J. Reimer M.M.

DOI: 10.3389/fncel.2017.00284

Website
http://www.crt-dresden.de/research/research-groups/core-groups/crtd-core-groups/...

Press Contact
Franziska Clauß, M.A.
Press Officer
Phone: +49 351 458 82065
E-Mail: franziska.clauss@tu-dresden.de

Franziska Clauß | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht New application for acoustics helps estimate marine life populations
16.01.2018 | University of California - San Diego

nachricht Unexpected environmental source of methane discovered
16.01.2018 | University of Washington Health Sciences/UW Medicine

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Scientists decipher key principle behind reaction of metalloenzymes

So-called pre-distorted states accelerate photochemical reactions too

What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...

Im Focus: The first precise measurement of a single molecule's effective charge

For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.

Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...

Im Focus: Paradigm shift in Paris: Encouraging an holistic view of laser machining

At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.

No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...

Im Focus: Room-temperature multiferroic thin films and their properties

Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.

Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...

Im Focus: A thermometer for the oceans

Measurement of noble gases in Antarctic ice cores

The oceans are the largest global heat reservoir. As a result of man-made global warming, the temperature in the global climate system increases; around 90% of...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

10th International Symposium: “Advanced Battery Power – Kraftwerk Batterie” Münster, 10-11 April 2018

08.01.2018 | Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

 
Latest News

Gran Chaco: Biodiversity at High Risk

17.01.2018 | Ecology, The Environment and Conservation

Only an atom thick: Physicists succeed in measuring mechanical properties of 2D monolayer materials

17.01.2018 | Physics and Astronomy

Fraunhofer HHI receives AIS Technology Innovation Award 2018 for 3D Human Body Reconstruction

17.01.2018 | Awards Funding

VideoLinks
B2B-VideoLinks
More VideoLinks >>>