Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Introduction of a novel system for in vitro analyses of zebrafish oligodendrocyte progenitor cells

23.10.2017

Introduction of a novel, easy-to-use and highly reproducible culture system for in vitro analyses of zebrafish oligodendrocyte progenitor cells (OPCs)

Dr. Michell M. Reimer, group leader at the Center for Regenerative Therapies Dresden (CRTD), Cluster of Excellence at the TU Dresden, and his team introduce a novel, easy-to-use, and highly reproducible OPC culture platform for adult zebrafish cells.


Dr. Michell M. Reimer

© CRTD


Main steps to obtain a highly pure adult zebrafish spinal oligodendrocyte progenitor cell (OPC) population

2017 Kroehne, Tsata, Marrone, Froeb, Reinhardt, Gompf, Dahl, Sterneckert and Reimer

This system will help to unravel the molecular and cellular programs that enable zebrafish to functionally regenerate spinal cord injuries. The results of this study have been published in the scientific journal Frontiers in Cellular Neuroscience.

Spinal cord injuries result from a blunt or penetrating trauma. This is generally caused by accidents that occur during sport activities or when driving. Injuries of the spinal cord can lead to extreme pain (e.g. pressure in the head, neck or back), the loss of sensation (e.g. in fingers or feet), the loss of control over different parts of the body, an abnormal sense of balance and many other symptoms.

According to the World Health Organization (WHO)*, as many as 500,000 people suffer from spinal cord injuries each year*. Humans do not regain spinal cord function after injury. However, zebrafish have the remarkable ability to functionally recover from spinal cord injury. They repair injured connections, replace damaged motor neurons and oligodendrocytes, enabling them to regain full movement within six weeks after injury.

The study introduced here focused on a population of support cells in the spinal cord that helps to protect surviving nerve cells (neurons) after injury: oligodendrocytes and their precursor cells. Oligodendrocytes, the cells that are known to produce the myelin sheaths which enable saltatory conduction of action potentials along the myelinated axons, are modulators of signal transmission along neuronal connections (axons) and also promote neuronal survival by providing metabolic support.

Oligodendrocyte death, occurring after a spinal cord injury, activates a process called de-myelination that results first in damage to surviving neuronal connections and finally in death of the affected neurons. Although lost mature oligodendrocytes can principally be replaced by resident oligodendrocyte progenitor cells (OPCs) this does not happen sufficiently enough in the human spinal cord after injury. Improving recruitment, activation and differentiation of OPCs is therefore hypothesised to improve functional outcome after a spinal cord injury in humans.

Here Dr. Reimer and his team asked the question, ‘what happens to mature oligodendrocytes after a spinal cord injury in adult zebrafish?’. They found that, like in humans, oligodendrocytes near a spinal cord injury site are massively lost within a week. However, two weeks after injury they found that the oligodendrocyte population was largely re-established, showing the remarkable regenerative capacity of the adult zebrafish spinal cord.

These results placed the resident OPC population in the focus of interest: what are the signals that control and enable the activation of these precursor cells in the adult zebrafish spinal cord? Dr. Reimer and his team decided to establish a novel in vitro platform to analyse zebrafish OPCs independently of the body, as this enables better control over the cells and opens up the possibility for novel methods of analysis. They developed a streamlined and fast, though inexpensive, method that allows direct access to a pure and vital population of zebrafish OPCs in less than 2 hours.

This simple protocol is based on automated fluorescent activated cell sorting (FACS) of OPCs. Using novel culture conditions Dr. Reimer’s team has shown it is now possible to maintain the cells for 16 days in vitro. Finally, they demonstrated that zebrafish OPCs differentiate into mature oligodendrocytes when cultured together with human motor neurons, differentiated from induced pluripotent stem cells. This shows that the basic mechanisms of oligodendrocyte differentiation are conserved across species and that understanding the regulation of zebrafish OPCs can contribute to the development of new treatment for human diseases.

As a next step, Dr. Reimer’s research team intend to analyse the effect of different drugs on zebrafish OPCs in order to potentially identify a method to improve functional spinal cord repair in humans.

Before becoming a research group leader at the CRTD in 2014 (for Regulation of developmental and regenerative processes in the spinal cord), the biologist Michell Reimer worked as a Post-Doctoral Fellow at the Centre for Neuroregeneration and the Centre for Cognitive and Neural Systems at the University of Edinburgh (UK) since 2009. From 2005-2008, Michell Reimer completed his PhD in the field of neuroscience at the Centre for Neuroscience Research, University of Edinburgh.

*http://www.who.int/mediacentre/news/releases/2013/spinal-cord-injury-20131202/en...

Publication
Title: Primary Spinal OPC Culture System from Adult Zebrafish to Study Oligodendrocyte Differentiation In Vitro
Kroehne V., Tsata V., Marrone L., Fröb C., Reinhardt S., Gompf A., Dahl A., Sterneckert J. Reimer M.M.

DOI: 10.3389/fncel.2017.00284

Website
http://www.crt-dresden.de/research/research-groups/core-groups/crtd-core-groups/...

Press Contact
Franziska Clauß, M.A.
Press Officer
Phone: +49 351 458 82065
E-Mail: franziska.clauss@tu-dresden.de

Franziska Clauß | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht Designer cells: artificial enzyme can activate a gene switch
22.05.2018 | Universität Basel

nachricht Flow of cerebrospinal fluid regulates neural stem cell division
22.05.2018 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: LZH showcases laser material processing of tomorrow at the LASYS 2018

At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.

At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...

Im Focus: Self-illuminating pixels for a new display generation

There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?

At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...

Im Focus: Explanation for puzzling quantum oscillations has been found

So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics

Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...

Im Focus: Dozens of binaries from Milky Way's globular clusters could be detectable by LISA

Next-generation gravitational wave detector in space will complement LIGO on Earth

The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...

Im Focus: Entangled atoms shine in unison

A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.

The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Save the date: Forum European Neuroscience – 07-11 July 2018 in Berlin, Germany

02.05.2018 | Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

 
Latest News

Designer cells: artificial enzyme can activate a gene switch

22.05.2018 | Life Sciences

PR of MCC: Carbon removal from atmosphere unavoidable for 1.5 degree target

22.05.2018 | Earth Sciences

Achema 2018: New camera system monitors distillation and helps save energy

22.05.2018 | Trade Fair News

VideoLinks
Science & Research
Overview of more VideoLinks >>>