Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Combatting hospital-acquired infections with protein metal complex

05.03.2014

A protein containing a metal complex for blue paint inhibits growth of a pathogenic bacterium through iron deprivation

Nagoya, Japan – Professor Yoshihito Watanabe (WPI-ITbM, Cooperating Researcher), Associate Professor Osami Shoji, Ms. Chikako Shirataki of Nagoya University and co-workers have found a new method using an artificial metalloprotein (a protein that contains a metal) to inhibit the growth of Pseudomonas aeruginosa bacteria, which is a common bacterium that can cause diseases in humans and evolves to exhibit multiple antibiotic resistance.


Figure 1. Heme iron capturing mechanism of P. aeruginosa bacteria by HasA protein.

Copyright : Nagoya University


Figure 2. Inhibition of heme iron uptake of P. aeruginosa by phthalocyanine-bound HasA protein.

Copyright : Nagoya University

The inhibition of growth has been achieved through the deprivation of iron uptake using an artificial metalloprotein. The study published in the online Early View on February 7, 2014 of Angewandte Chemie International Edition, is expected to bring hope in the battle against bacteria.

P. aeruginosa bacteria exists in many aquatic areas and is prevalent in hospitals. Although they do not usually affect healthy people, they increase the risk for infection of patients with low immunity. Their high resistance towards many antibiotics makes complete elimination of them extremely difficult. Like humans, bacteria require the uptake of heme iron for their survival, and a protein (HasA) is secreted from bacteria to capture heme from its host. The heme-bound HasA protein transfers heme via receptor proteins on the cell surface of the bacterium, P. aeruginosa (Figure 1).

“Upon looking closely at the crystal structure of the HasA protein binding heme, we considered the possibility of the HasA protein to bind to a metal complex that has a similar structure as heme” says Associate Professor Osami Shoji, who led the study. “We found synthetic metal complexes that can mimic heme and bind to the HasA protein. To our delight, one of the resulting complexes successfully inhibited growth of P. aeruginosa bacteria.” 

“It took us around four years to discover that phthalocyanine, which is a blue paint used on the surface of the Japanese bullet trains and road signs, could bind competitively to the HasA protein”, adds Ms. Chikako Shirataki, a PhD student in her final year, “crystal structures of metal protein complexes helped us to show that the phthalocyanine-bound HasA protein blocks the receptors on the cell surface of the bacterium and thus, inhibits the uptake of heme.” When bacteria are deprived of iron, further growth of the bacteria is inhibited (Figure 2).

P. aeruginosa infections can potentially lead to pneumonia and an effective treatment method is highly required. This finding by Shoji’s group opens new doors to treat P. aeruginosa infections by using an unprecedented approach to inhibit the growth of bacteria. Associate Professor Shoji states, “With the advice of medical doctors, we are currently working to develop a new system to wipe out bacteria by tuning various metal complexes. Although the efficiency is not high yet, we have already established a mechanism to eliminate bacteria and we are considering how to apply it to different cases.”

ichi Ozaki, Hiroshi Sugimoto, Yoshitsugu Shiro, Yoshihito Watanabe, is published in the Early View on February 7, 2014 in Angewandte Chemie International Edition. The article was selected as an inside cover. DOI: 10.1002/anie.201307889

This work was conducted with Mitsuyoshi Terada of Nagoya University, Professor Shin-ichi Ozaki of Yamaguchi University, Dr. Hiroshi Sugimoto and Professor Yoshitsugu Shiro of RIKEN SPring-8 Center, Harima Institute.

Author Contact
Associate Professor Osami Shoji
Department of Chemistry, Graduate School of Science, Nagoya University
Furo-Cho, Chikusa-ku, Nagoya 464-8602, Japan
TEL/FAX: +81-52-789-3557
E-mail: osami.shoji@a.mbox.nagoya-u.ac.jp

Public Relations Contact
Dr. Ayako Miyazaki
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University
Furo-Cho, Chikusa-ku, Nagoya 464-8601, Japan
TEL: +81-52-789-4999 FAX: +81-52-789-3240
E-mail: ayako.miyazaki@itbm.nagoya-u.ac.jp

Nagoya University Public Relations Office TEL: +81-52-789-2016 FAX: +81-52-788-6272
E-mail: kouho@post.jimu.nagoya-u.ac.jp

Associated links

Journal information

Angewandte Chemie International Edition

Ayako Miyazaki | Research SEA

Further reports about: aeruginosa artificial bacteria bacterium infections resistance structure

More articles from Life Sciences:

nachricht New switch decides between genome repair and death of cells
27.09.2016 | University of Cologne - Universität zu Köln

nachricht A blue stoplight to prevent runaway photosynthesis
27.09.2016 | National Institute for Basic Biology

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New welding process joins dissimilar sheets better

Friction stir welding is a still-young and thus often unfamiliar pressure welding process for joining flat components and semi-finished components made of light metals.
Scientists at the University of Stuttgart have now developed two new process variants that will considerably expand the areas of application for friction stir welding.
Technologie-Lizenz-Büro (TLB) GmbH supports the University of Stuttgart in patenting and marketing its innovations.

Friction stir welding is a still-young and thus often unfamiliar pressure welding process for joining flat components and semi-finished components made of...

Im Focus: First quantum photonic circuit with electrically driven light source

Optical quantum computers can revolutionize computer technology. A team of researchers led by scientists from Münster University and KIT now succeeded in putting a quantum optical experimental set-up onto a chip. In doing so, they have met one of the requirements for making it possible to use photonic circuits for optical quantum computers.

Optical quantum computers are what people are pinning their hopes on for tomorrow’s computer technology – whether for tap-proof data encryption, ultrafast...

Im Focus: OLED microdisplays in data glasses for improved human-machine interaction

The Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP has been developing various applications for OLED microdisplays based on organic semiconductors. By integrating the capabilities of an image sensor directly into the microdisplay, eye movements can be recorded by the smart glasses and utilized for guidance and control functions, as one example. The new design will be debuted at Augmented World Expo Europe (AWE) in Berlin at Booth B25, October 18th – 19th.

“Augmented-reality” and “wearables” have become terms we encounter almost daily. Both can make daily life a little simpler and provide valuable assistance for...

Im Focus: Artificial Intelligence Helps in the Discovery of New Materials

With the help of artificial intelligence, chemists from the University of Basel in Switzerland have computed the characteristics of about two million crystals made up of four chemical elements. The researchers were able to identify 90 previously unknown thermodynamically stable crystals that can be regarded as new materials. They report on their findings in the scientific journal Physical Review Letters.

Elpasolite is a glassy, transparent, shiny and soft mineral with a cubic crystal structure. First discovered in El Paso County (Colorado, USA), it can also be...

Im Focus: Complex hardmetal tools out of the 3D printer

For the first time, Fraunhofer IKTS shows additively manufactured hardmetal tools at WorldPM 2016 in Hamburg. Mechanical, chemical as well as a high heat resistance and extreme hardness are required from tools that are used in mechanical and automotive engineering or in plastics and building materials industry. Researchers at the Fraunhofer Institute for Ceramic Technologies and Systems IKTS in Dresden managed the production of complex hardmetal tools via 3D printing in a quality that are in no way inferior to conventionally produced high-performance tools.

Fraunhofer IKTS counts decades of proven expertise in the development of hardmetals. To date, reliable cutting, drilling, pressing and stamping tools made of...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

European Health Forum Gastein 2016 kicks off today

28.09.2016 | Event News

Laser use for neurosurgery and biofabrication - LaserForum 2016 focuses on medical technology

27.09.2016 | Event News

Experts from industry and academia discuss the future mobile telecommunications standard 5G

23.09.2016 | Event News

 
Latest News

New imaging technique in Alzheimer’s disease - opens up possibilities for new drug development

28.09.2016 | Medical Engineering

Innovate coating extends the life of materials for industrial use

28.09.2016 | Materials Sciences

Blockchain Set to Transform the Financial Services Market

28.09.2016 | Business and Finance

VideoLinks
B2B-VideoLinks
More VideoLinks >>>