New technique for sorting biomolecules could lead to efficient clinical diagnostics and chemical purification
Employing an ingenious microfluidic design that combines chemical and mechanical properties, a team of Harvard scientists has demonstrated a new way of detecting and extracting biomolecules from fluid mixtures. The approach requires fewer steps, uses less energy, and achieves better performance than several techniques currently in use and could lead to better technologies for medical diagnostics and chemical purification.
The biomolecule sorting technique was developed in the laboratory of Joanna Aizenberg, Amy Smith Berylson Professor of Materials Science at Harvard School of Engineering and Applied Sciences (SEAS) and Professor in the Department of Chemistry and Chemical Biology. Aizenberg is also co-director of the Kavli Institute for Bionano Science and Technology and a core faculty member at Harvard's Wyss Institute for Biologically Inspired Engineering, leading the Adaptive Materials Technologies platform there.
The new microfluidic device, described in a paper appearing today in the journal Nature Chemistry, is composed of microscopic "fins" embedded in a hydrogel that is able to respond to different stimuli, such as temperature, pH, and light. Special DNA strands called aptamers, that under the right conditions bind to a specific target molecule, are attached to the fins, which move the cargo between two chemically distinct environments. Modulating the pH levels of the solutions in those environments triggers the aptamers to "catch" or "release" the target biomolecule.
After using computer simulations to test their novel approach, in collaboration with Prof. Anna C. Balazs from the University of Pittsburgh, Aizenberg's team conducted proof-of-concept experiments in which they successfully separated thrombin, an enzyme in blood plasma that causes the clotting of blood, from several mixtures of proteins. Their research suggests that the technique could be applicable to other biomolecules, or used to determine chemical purity and other characteristics in inorganic and synthetic chemistry.
"Our adaptive hybrid sorting system presents an efficient chemo-mechanical transductor, capable of highly selective separation of a target species from a complex mixture--all without destructive chemical modifications and high-energy inputs," Aizenberg said. "This new approach holds promise for the next-generation, energy-efficient separation and purification technologies and medical diagnostics."
The system is dynamic; its integrated components are highly tunable. For example, the chemistry of the hydrogel can be modified to respond to changes in temperature, light, electric and magnetic fields, and ionic concentration. Aptamers, meanwhile, can target a range of proteins and molecules in response to variations in pH levels, temperature, and salt.
"The system allows repeated processing of a single input solution, which enables multiple recycling and a high rate of capture of the target molecules," said lead author Ximin He, Assistant Professor of Materials Science and Engineering at Arizona State University and formerly a postdoctoral research fellow in Aizenberg's group at Harvard.
Conventional biomolecule sorting systems rely on external electric fields, infrared radiation, and magnetic fields, and often require chemical modifications of the biomolecules of interest. That means setups can be used only once or require a series of sequential steps. In contrast, said Ankita Shastri, a graduate student in Chemistry and Chemical Biology at Harvard and a member of Aizenberg's group, the new catch-transport-and-release system "is more efficient--requiring minimal steps and less energy, and effective--achieving recovery of almost all of the target biomolecule through its continuous reusability."
The authors say that the system could provide a means of removing contaminants from water--and even be tailored to enable energy-efficient desalination of seawater. It could also be used to capture valuable minerals from fluid mixtures.
Other contributors to the work include Lynn M. McGregor and Yolanda Vasquez from Harvard University; Ya Liu, Amitabh Bhattacharya, Yongting Ma, and Olga Kuksenok from the University of Pittsburgh; Valerie Harris, Hanqing Nan, and Maritza Mujica from Arizona State University; and Michael Aizenberg from the Wyss Institute.
The research was supported by the U.S. Department of Energy (DE-SC0005247).
Paul Karoff | EurekAlert!
Study identifies RNA molecule that shields breast cancer stem cells from immune system
23.05.2017 | Princeton University
“Pregnant” Housefly Males Demonstrate the Evolution of Sex Determination
23.05.2017 | Universität Zürich
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...
In the race to produce a quantum computer, a number of projects are seeking a way to create quantum bits -- or qubits -- that are stable, meaning they are not much affected by changes in their environment. This normally needs highly nonlinear non-dissipative elements capable of functioning at very low temperatures.
In pursuit of this goal, researchers at EPFL's Laboratory of Photonics and Quantum Measurements LPQM (STI/SB), have investigated a nonlinear graphene-based...
Dental plaque and the viscous brown slime in drainpipes are two familiar examples of bacterial biofilms. Removing such bacterial depositions from surfaces is...
23.05.2017 | Event News
22.05.2017 | Event News
17.05.2017 | Event News
23.05.2017 | Earth Sciences
23.05.2017 | Life Sciences
23.05.2017 | Physics and Astronomy