Small regulatory RNA molecules are vital for salmonella and other bacteria potentially harmful to humans: This RNA type controls gene activity and allows bacteria to quickly adjust to changing conditions of living and stress as are typical during an infection, for example, when entering the blood stream or inside human cells.
Professor Jörg Vogel, head of the Institute for Molecular Infection Biology of the Julius-Maximilians-Universität Würzburg (JMU) in Bavaria, Germany, is a pioneer in researching small regulatory RNA molecules. He and his team are determined to get to the bottom of how these molecules work and act. His works could also show new ways to fight pathogens.
ProQ binds nearly 100 regulatory RNAs
New findings from Vogel's team have now been published in the journal PNAS: So far, two proteins (Hfg and CsrA) have been known to bind closely to the bacteria's regulatory RNA molecules and influence their activities. Using a new self-designed method, the Würzburg team has now discovered a long-suspected third protein (ProQ) whose function inside the cell has been unknown until recently.
Experiments showed that the ProQ protein binds to 98 regulatory RNAs of the enterobacterial Salmonella enterica. The bacterium has been found to have around 300 such RNAs in total. Moreover, ProQ seems to have specialised in RNA molecules with a rather complex structure.
This protein and the RNA molecules that bind to it represent a largely unresearched class of gene activity regulators in the bacterial "RNA universe". "It will be particularly exciting to find out how ProQ is able to pinpoint the highly structured RNAs among millions of other RNA molecules in a cell," says Jörg Vogel.
PNAS considers results significant
These results have been reported in PNAS by the Würzburg professor together with Alexandre Smirnov, Konrad Förstner, Erik Holmqvist and Regina Günster as well as colleagues from Greifswald and Cologne. Considered highly significant for bacterial research, the new findings are featured in the journal's "Research Highlight" section.
A new technique developed by the JMU team has successfully tracked down the activities of the ProQ protein. "The methods available so far were subject to certain limits with regard to detecting and generally classifying RNA protein interactions which we have overcome here," Professor Vogel further. Since the new method can basically be applied to any other organism, it is expected to provide more progress in researching regulatory RNA.
Dr. Jörg Vogel | EurekAlert!
Embryonic development: How do limbs develop from cells?
18.05.2018 | Humboldt-Universität zu Berlin
Reading histone modifications, an oncoprotein is modified in return
18.05.2018 | American Society for Biochemistry and Molecular Biology
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...
A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.
The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...
Cardiovascular tissue engineering aims to treat heart disease with prostheses that grow and regenerate. Now, researchers from the University of Zurich, the Technical University Eindhoven and the Charité Berlin have successfully implanted regenerative heart valves, designed with the aid of computer simulations, into sheep for the first time.
Producing living tissue or organs based on human cells is one of the main research fields in regenerative medicine. Tissue engineering, which involves growing...
A team of scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg investigated optically-induced superconductivity in the alkali-doped fulleride K3C60under high external pressures. This study allowed, on one hand, to uniquely assess the nature of the transient state as a superconducting phase. In addition, it unveiled the possibility to induce superconductivity in K3C60 at temperatures far above the -170 degrees Celsius hypothesized previously, and rather all the way to room temperature. The paper by Cantaluppi et al has been published in Nature Physics.
Unlike ordinary metals, superconductors have the unique capability of transporting electrical currents without any loss. Nowadays, their technological...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
18.05.2018 | Power and Electrical Engineering
18.05.2018 | Information Technology
18.05.2018 | Information Technology