Waterhemp has been locked in an arms race with farmers for decades. Nearly every time farmers attack the weed with a new herbicide, waterhemp becomes resistant to it, reducing or eliminating the efficacy of the chemical. Some waterhemp populations have evolved resistance to multiple herbicides, making them incredibly difficult to kill.
Adding to the challenge is the fact that waterhemp can evolve resistance in at least two ways. In target-site resistance, a gene mutation changes the protein that the herbicide is designed to attack. With an ill-fitting protein binding site, the herbicide becomes ineffective. The plus side of target-site resistance is that it is relatively easy to identify using standard lab procedures.
Metabolic resistance is a different beast. Plants use any one of hundreds of possible enzymes to detoxify the chemical, rendering it useless. Although University of Illinois weed scientists Dean Riechers, Rong Ma, and Josh Skelton recently developed a quick and easy test for metabolic resistance, they still had no way of knowing which enzyme was responsible.
"We think we found the needle in the haystack," Riechers says.
Riechers and his colleagues focused on metabolic resistance to atrazine, a chemical that has been used for decades and is still sprayed on approximately 80 percent of the corn acreage in the United States, despite increasing resistance and concerns about environmental impacts.
From previous research at U of I, the team knew that resistant waterhemp metabolizes atrazine with a class of enzymes known as GSTs. "Plants are known to have anywhere from 50-120 GST genes. We wanted to know if resistance was coming from just one of those, and whether we could find it," Riechers says.
Former graduate student Anton Evans isolated candidate GST proteins from waterhemp and looked at their expression in resistant and sensitive plants. One of the GST proteins was extremely abundant in resistant plants, but almost nonexistent in sensitive plants. Graduate student Sarah O'Brien looked more closely at the gene encoding that GST protein, and, in particular, at its variants or alleles. She noticed that when two dominant alleles for this gene were present, it took more than 14 times the recommended atrazine rate to damage the plants.
"The heterozygous plants -- those with just one copy of the dominant allele -- had much higher injury. In an experiment with four or five heterozygous plants, two or three would die and the others were stunted and had a lot of dead tissue. But the homozygous resistant plants -- those with two copies of the dominant allele - almost looked like they hadn't been sprayed," O'Brien says.
"Plants without the dominant allele just got hammered, even at low herbicide application rates," Riechers adds.
This evidence, along with more detailed molecular data, gives the team confidence that they have discovered something unique. "This may be the only case where we've actually found the gene responsible for metabolic resistance in a broadleaf weed, as opposed to target-site resistance," Riechers explains, "for any herbicide."
Going forward, the test for this gene will be similar to the test for target-site atrazine resistance. The new information also could be used by industry to develop new chemicals to control waterhemp.
"As long as we know the gene, you could potentially knock it out and make the plant sensitive again. You could design a GST-inhibiting chemical that's specific to this one GST," Riechers says.
The article, "Biochemical characterization of metabolism-based atrazine resistance in Amaranthus tuberculatus and identification of an expressed GST associated with resistance," is published in Plant Biotechnology Journal. Anton Evans Jr., Sarah O'Brien, Rong Ma, Aaron Hager, Chance Riggins, Kris Lambert, and Dean Riechers contributed to the paper. Syngenta U.K. supported the project.
Lauren Quinn | EurekAlert!
New study shows producers where and how to grow cellulosic biofuel crops
17.01.2018 | University of Illinois College of Agricultural, Consumer and Environmental Sciences
Robotic weeders: to a farm near you?
10.01.2018 | American Society of Agronomy
On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.
We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...
What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...
For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.
Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...
At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.
No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...
Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.
Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...
08.01.2018 | Event News
11.12.2017 | Event News
08.12.2017 | Event News
19.01.2018 | Materials Sciences
19.01.2018 | Health and Medicine
19.01.2018 | Physics and Astronomy