Discovering new data on barley domestication
An international team of researchers has succeeded for the first time in sequencing the genome of Chalcolithic barley grains. This is the oldest plant genome to be reconstructed to date. The 6,000 years old seeds were retrieved from Yoram Cave in the southern cliff of Masada fortress in the Judean Desert in Israel, close to the Dead Sea. Genetically, the prehistoric barley is very similar to present-day barley grown in the Southern Levant supporting the existing hypothesis of barley domestication having occurred in the Upper Jordan Valley.
Right: Photograph during excavation – note the excellent dry preservation of plant remains Left: Photograph of a well-preserved, desiccated barley grain found at Yoram Cave.
Uri Davidovich/ Credit: Nature genetics, DOI: 10.1038/ng.3611
The members of the research team came from the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) in Gatersleben, Germany; Bar-Ilan University in Ramat Gan, Israel; Hebrew University, Jerusalem, Israel; the Max Planck Institute for the Science of Human History in Jena, Germany; and the University of Haifa, Israel; The James Hutton Institute, UK; University of California, Santa Cruz, USA; University of Minnesota St. Paul, USA; University of Tübingen, Germany.
The analyzed grains, together with tens of thousands of other plant remains, were retrieved during a systematic archaeological excavation headed by Uri Davidovich, from the Institute of Archaeology, The Hebrew University of Jerusalem, and Nimrod Marom, from Zinman Institute of Archaeology, University of Haifa, Israel. The archaeobotanical analysis was led by Ehud Weiss, of Bar-Ilan University. The cave is very difficult to access and was used only for a short time by humans, some 6,000 years ago, probably as ephemeral refuge.
Oldest plant genome reconstructed to date:
Most examination of archaeobotanical findings has been limited to the comparison of ancient and present-day specimens based on their morphology. Up to now, only prehistoric corn has been genetically reconstructed. In this research, the team succeeded in sequencing the complete genome of the 6,000-year-old barley grains. The results are now published in the online version of the journal Nature Genetics.
"These archaeological remains provided a unique opportunity for us to finally sequence a Chalcolithic plant genome. The genetic material has been well-preserved for several millennia due to the extreme dryness of the region," explains Weiss. In order to determine the age of the ancient seeds, the researchers split the grains and subjected half of them to radiocarbon dating while the other half was used to extract the ancient DNA. “For us, ancient DNA works like a time capsule that allows us to travel back in history and look into the domestication of crop plants at distinct time points in the past,” explains Johannes Krause, Director of the Department of Archaeogentics at the Max Planck Institute for the Science of Human History in Jena. The genome of Chalcolithic barley grains is the oldest plant genome to be reconstructed to date.
Domestication of barley completed very early:
Wheat and barley were already grown 10,000 years ago in the Fertile Crescent, a sickle-shaped region stretching from present-day Iraq and Iran through Turkey and Syria into Lebanon, Jordan and Israel. Up to this day, the wild forms of these two crops persist in the region and are among the major model species studied at the Institute of Evolution in the University of Haifa. “It was from there that grain farming originated and later spread to Europe, Asia and North Africa” explains Tzion Fahima, of the University of Haifa.
“Our analyses show that the seeds cultivated 6,000 years ago greatly differ genetically from the wild forms we find today in the region. However, they show considerable genetic overlap with present-day domesticated lines from the region,” explains Nils Stein, who directed the comparison of the ancient genome with modern genomes at the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, with the support Robbie Waugh and colleagues at the James Hutton Institute, Dundee, Scotland, and Gary Muehlbauer, University of Minnesota, USA. "This demonstrates that the domestication of barley in the Fertile Crescent was already well advanced very early."
The comparison of the ancient seeds with wild forms from the region and with so-called ‘landraces’ (i.e., local barley lines grown by farmers in the Near East) enabled to geographically suggest, according to Tzion Fahima and his colleagues at the University of Haifa and Israel's Tel-Hai College, “the origin of the domestication of barley within the Upper Jordan Valley – a hypothesis that is also supported by two archaeological sites in the surrounding area where the hitherto earliest remains of barley cultivation have been found”
Immigrants “trust” in extant landraces:
Also the genetic overlap with present-day domesticated lines from the region is revealing to the researchers. “This similarity is an amazing finding considering to what extent the climate, but also the local flora and fauna, as well as the agricultural methods, have changed over this long period of time,” says Martin Mascher, from the Leibniz Institute of Plant Genetics and Crop Plant Research, the lead author of the study. The researchers therefore assume that conquerors and immigrants coming to the region did not bring their own crop seeds from their former homelands, but continued cultivating the locally adapted extant landraces.
New insights into the origins of our crop plants:
By combining archaeology, archaeobotany, genetics and computational genomics in an interdisciplinary study has produced novel insights into the origins of our crop plants. “This is just the beginning of a new and exciting line of research,” predicts Verena Schuenemann, from Tuebingen University, the second lead author of the study. “DNA-analysis of archaeological remains of prehistoric plants will provide us with novel insights into the origin, domestication and spread of crop plants.”
Martin Mascher, Verena J. Schuenemann, Uri Davidovich, Nimrod Marom, Axel Himmelbach, Sariel Hübner, Abraham Korol, Michal David, Ella Reiter, Simone Riehl, Mona Schreiber, Samuel H. Vohr, Richard E. Green, Ian K. Dawson, Joanne Russell, Benjamin Kilian, Gary J. Muehlbauer, Robbie Waugh, Tzion Fahima, Johannes Krause, Ehud Weiss, Nils Stein (2016) Genomic analysis of 6,000-year-old cultivated grain illuminates the domestication history of barley. Nature Genetics. DOI: 10.1038/ng.3611
Dr. Nils Stein
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Prof. Johannes Krause
Max Planck Institute for the Science of Human History (MPI-SHH)
Kahlaische Str. 10,
07745 Jena, Germany
+49 3641 686-600
Max Planck Institute for the Science of Human History
Kahlaische Str. 10,
07745 Jena, Germany,
+49 3641 686-960
Dr. Sabine Odparlik
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3, OT Gatersleben
06466 Stadt Seeland, Germany
+49 39482 5427
Dr. Sabine Odparlik | idw - Informationsdienst Wissenschaft
Ammonium nitrogen input increases the synthesis of anticarcinogenic compounds in broccoli
26.04.2017 | University of the Basque Country
New data unearths pesticide peril in beehives
21.04.2017 | Cornell University
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
28.04.2017 | Event News
20.04.2017 | Event News
18.04.2017 | Event News
28.04.2017 | Medical Engineering
28.04.2017 | Earth Sciences
28.04.2017 | Life Sciences