fadeAWAY: Highly effective Anti-fading agent for single-molecule fluorescence microscopy with extend lifetime
Single-molecule fluorescence provides valuable
information on elementary biological processe s in a range of fluorescence imaging, microscopy and spectroscopy methods. Fluorescence spectroscopy relies to a large extent on extraordinary bright and photostable organic chromophores. However, fading (photobleaching) of fluorescent dyes and blinking of fluorochromes causes significant problems in these applications. While some anti-fading products (e.g. Trolox) are commercially available, they provide only limited improvements in fading and blinking. As a result, many applications of fluorescence methods (e.g. particular biological and immunofluorescence studies) are still not possible.
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