Visualizing virus replication in 3 dimensions

Dengue fever is the most common infectious disease transmitted by mosquitoes – some 100 million people around the world are infected. Researchers at the Hygiene Institute at Heidelberg University Hospital were the first to present a three-dimensional model of the location in the human cell where the virus is reproduced.

Their research provides an insight into the exact process of viral replication and serves as a model for other viruses whose replication is still unclear, such as the hepatitis C virus. In addition, it offers new approaches for developing measures to prevent or treat dengue fever. Up to now, neither a vaccine nor a specific antiviral therapy exists.

Professor Dr. Ralf Bartenschlager, director of the Department of Molecular Virology at the Heidelberg Hygiene Institute and his team, working in cooperation with colleagues from the European Molecular Biology Laboratory (EMBL) have published their study in the latest issue of the prestigious journal Cell Host & Microbes.

Viruses do not have a metabolism and cannot produce proteins from their genetic material (RNA or DNA) on their own. They can replicate only inside a host cell – but where and how exactly does this take place? The answer to this question is crucial for developing therapy.

Viruses transform human cell membranes for their purposes

Dengue viruses reproduce in what is known as the endoplasmic reticulum, a membrane network interconnected with the nuclear envelope; this is where proteins are synthesized. The dengue virus uses this membrane network and transforms it for its own use.

“We now know that viral RNA is replicated in vesicles in the endoplasmic reticulum and is secreted through tiny pores. We were also able to show that replication of the virus genome and its encapsulation in new virus particles are directly linked,” said Professor Bartenschlager. The new virus genomes are secreted through pores into the intracellular space where they are incorporated into pre-stages of viruses and then penetrate the endoplasmic reticulum a second time. There they are enveloped in a membrane that disguises them for the cell so that they can be secreted like normal cellular material. The reproduction cycle can begin again.

References:

Sonja Welsch, Sven Miller, Ines Romero-Brey, Andreas Merz, Christopher
Bleck, Paul Walther, Stephen D. Fuller, Claude Antony, Jacomine Krijnse-Locker, Ralf Bartenschlager, Composition and Three-Dimensional Architecture of the Dengue Virus Replication and Assembly Sites, Cell Host & Microbes 2009, 5, 4.

Contact person:

Prof. Dr. Ralf Bartenschlager
Department of Molecular Virology
Heidelberg University School of Medicine
Im Neuenheimer Feld 345
69120 Heidelberg
Tel.: 06221 56-4569
E-mail: Ralf_Bartenschlager@med.uni-heidelberg.de
Heidelberg University Hospital and Medical Faculty:
Internationally recognized patient care, research, and teaching
Heidelberg University Hospital is one of the largest and most prestigious medical centers in Germany. The Medical Faculty of Heidelberg University belongs to the internationally most renowned biomedical research institutions in Europe. Both institutions have the common goal of developing new therapies and implementing them rapidly for patients. With about 7,000 employees, training and qualification is an important issue. Every year, around 500,000 patients are treated on an inpatient or outpatient basis in more than 40 clinics and departments with 1,600 beds. Currently, about 3,100 future physicians are studying in Heidelberg; the reform Heidelberg Curriculum Medicinale (HeiCuMed) is one of the top medical training programs in Germany.

Requests by journalists:
Dr. Annette Tuffs
Head of Public Relations and Press Department
University Hospital of Heidelberg and
Medical Faculty of Heidelberg
Im Neuenheimer Feld 672
D-69120 Heidelberg
Germany
phone: +49 6221 / 56 45 36
fax: +49 6221 / 56 45 44
e-mail: annette.tuffs(at)med.uni-heidelberg.de

Media Contact

Dr. Ralf Bartenschlager EurekAlert!

All latest news from the category: Life Sciences and Chemistry

Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.

Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.

Back to home

Comments (0)

Write a comment

Newest articles

Digital ICTM Conference 2022

Achieving climate targets in turbomachinery engineering through digitization. Manufacturers and suppliers of engines and stationary turbomachinery have long recognized that the overriding goals of conserving resources and reducing emissions can…

Creating a reference map to explore the electronic device mimicking brain activity

Maps are essential for exploring trackless wilderness or vast expanses of ocean. The same is true for scientific studies that try to open up new fields and develop brand-new devices….

Arase satellite uncovers coupling between plasma waves and charged particles in Geospace

Scientists unravel a bit more of the mystery underlying how wave-particle interactions generate other plasma waves in Geospace. In a new study published in Physical Review Letters, researchers from Japan…

Partners & Sponsors