Screening for transformed human mesenchymal stromal cells with tumorigenic potential
Currently, MSCs are being widely investigated as a potential treatment for various diseases. According to ClinicalTrials.gov, over 350 clinical trials using MSCs have been registered by the end of 2013 (with a search of: mesenchymal stem cells).
For cell transplantation, MSCs are often isolated from either the patient or from a third party donor, and then expanded in cell culture before therapeutic application. In fact, spontaneous transformation of primary cells in cell culture has been well-investigated over decades. Malignant transformation of murine and monkey MSCs has also recently been reported.
The current study confirmed that spontaneous tumorigenic transformation of human MSCs can occur during cell culture expansion. This potentially has large implications for the clinical application of ex vivo expanded MSCs. “Although this transformation is rare, we do need to carefully examine the presence of these aberrant cells in MSC cultures, before transplanting into patients”, stresses the first author Dr. Pan. “We now have identified RNA molecule signatures that can be applied as a potential biomarker for the detection of these dangerous cells in long-term cultures”, said senior author Dr. van der Laan. “However, further research is required to validate this biomarker in clinical grade cultures of MSCs that are used in clinical trials”.
Dr. Steven R. Goodman, Editor-in-Chief of Experimental Biology and Medicine said “This study provides a possible method for testing the safety of expanded adult stem cells. We look forward to the validation of these RNA biomarkers”.
Experimental Biology and Medicine is a journal dedicated to the publication of multidisciplinary and interdisciplinary research in the biomedical sciences. The journal was first established in 1903. Experimental Biology and Medicine is the journal of the Society of Experimental Biology and Medicine. To learn about the benefits of society membership visit http://www.sebm.org. If you are interested in publishing in the journal please visit http://ebm.sagepub.com/.
Alle Nachrichten aus der Kategorie: Life Sciences
Articles and reports from the Life Sciences area deal with applied and basic research into modern biology, chemistry and human medicine.
Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.
Grow faster, die sooner: How growth rates influence the fitness of bacteria
“The fitness of bacteria is more complex than expected,” explains Ulrich Gerland, professor for the theory of complex biosystems at the Technical University of…
Spintronics: Researchers show how to make non-magnetic materials magnetic
In solid-state physics, oxide layers only a few nanometres thick are known to form a so-called two-dimensional electron gas. These thin layers, separated from…
Caterpillars of the wax moth love eating plastic: Fraunhofer LBF investigates degradation process
Within the Framework of a research project on the chemical imaging analysis of plastic digestion in caterpillars (RauPE), a team from Fraunhofer LBF used…