Can you evolve while being robust?

Claudia Igler, PhD student in the group of Calin Guet at IST Austria (C) by IST Austria, 2018

It is often thought that DNA, together with the genes encoded in it, is the essence of life. But equally important is coordinating when genes are turned on and off. In fact, it is this process, called regulation of gene expression, that defines life by allowing organisms to react to their surroundings rather than being static automatons.

As even the smallest organisms like bacteria have many genes, coordinating their expression is done by a dedicated set of proteins, which bind specific sites in the DNA (called ‘promoters’) in order to turn genes on or off. Each such pairing between a protein and its associated promoter constitutes one of myriad connections in the organismal gene regulatory network.

Gene regulatory networks are intricately tuned, so how can they evolve and change? In a study published today in Nature Ecology and Evolution, a team of researchers at the Institute of Science and Technology Austria (IST Austria), including co-first authors Claudia Igler (PhD student in Calin Guet’s group) and Mato Lagator (Postdoc in the Guet group), as well as Calin Guet, Gašper Tkačik and Jonathan Bollback (University of Liverpool), describes how individual regulatory connections can change over time.

Usually, gene regulatory networks are studied at the global level, with researchers seeking to understand how the properties of the network as a whole determine its evolution. Igler et al., however, decided to study network evolution from the local perspective in order to understand how connections in the network change. To do so, they used two DNA-binding proteins and their associated promoters. These proteins are called ‘repressors’, as their binding to DNA inhibits gene expression.

The researchers then introduced mutations into the promoters and observed how these changes affected the binding of repressors. Repressors can react to changes in two ways, Igler says: “A repressor can be robust, meaning that mutations do not affect it much since it maintains binding to a promoter despite the mutations. Alternatively, a repressor can be evolvable, which means that it readily responds to mutations by acquiring binding to new promoters. These two responses to mutations seem to be, by definition, mutually exclusive – a protein that is more robust to mutations ought to respond to mutations less, and should hence be less evolvable!”

But, as is often the case, biology is full of surprises. Comparing between the two studied repressors, the researchers found that the more robust repressor acquired binding to new promoters more readily.

By developing a biophysical model based on the thermodynamics of protein-DNA binding, the researchers were not only able to explain their surprising observations, but could generalize their findings, as Igler describes:

“How repressors react to mutations in their binding sites indicates how they can function within the regulatory network. One group of repressors, the local ones, are very specific – they bind only to a handful of promoters and do not acquire new binding easily. Another group of repressors, the global ones, is promiscuous and keeps on binding to their promoter even when it is heavily mutated, while also easily starting to bind new sites.”

Claudia Igler, one of the paper’s co-first authors, is a PhD student in the group of Calin Guet. IST Austria’s interdisciplinary graduate school offers fully-funded PhD positions in the natural and mathematical sciences. For Igler, the requirement to work with different groups in the initial rotation year was the graduate school’s strongest point:

“I come from a theoretical background in mathematical and biomedical engineering. However, I had always wanted to try working in a lab. IST Austria’s graduate school was ideal as the rotations allowed me to see whether experimental work fitted me. Although I had initially thought I’d work in neuroscience, I joined Calin Guet for my first rotation and liked it so much that I decided to stay here.” Applications for the next year of study at the IST Austria graduate school start in mid-October: http://phd.ist.ac.at

Media Contact

Bernhard Wenzl idw - Informationsdienst Wissenschaft

Alle Nachrichten aus der Kategorie: Life Sciences

Articles and reports from the Life Sciences area deal with applied and basic research into modern biology, chemistry and human medicine.

Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.

Zurück zur Startseite

Kommentare (0)

Schreib Kommentar

Neueste Beiträge

High-thermoresistant biopolyimides become water-soluble like starch

This is the first report for the syntheses of water-soluble polyimides which are Interestingly derived from bio-based resources, showing high transparency, tunable mechanical strength and the highest thermoresistance in water-soluble…

Land management in forest and grasslands

How much can we intensify? A first assessment of the effects of land management on the links between biodiversity, ecosystem functions and ecosystem services. Ecosystem services are crucial for human…

A molecular break for root growth

The dynamic change in root growth of plants plays an important role in their adjustment to soil conditions. Depending on the location, nutrients or moisture can be found in higher…

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.

Close