Bacterial lifestyle alters the evolution of antibiotic resistance
University of Pittsburgh School of Medicine researchers repeatedly exposed bacteria to the antibiotic ciprofloxacin to force rapid evolution. As expected, the bacteria developed resistance to the drug, but surprisingly, their lifestyle affected which specific adaptations emerged, according to a study published today in eLife.
“What we're simulating in the lab is happening in the wild, in the clinic, during the development of drug resistance,” said senior author Vaughn Cooper, Ph.D., director of the Center for Evolutionary Biology and Medicine at Pitt.
“Our results show that biofilm growth shapes the way drug resistance evolves.” According to study lead author Alfonso Santos-Lopez, Ph.D., a postdoctoral researcher in Cooper's lab, this finding could uncover vulnerabilities that may prove useful when treating drug-resistant infections.
“Antibiotic resistance is one of our main problems in medicine,” Santos-Lopez said. “We have to develop new treatments, and one idea is to take advantage of what the field calls 'collateral sensitivity.' When bacteria evolve resistance to one drug, it can expose a vulnerability to a different class of antibiotics that can effectively kill the bacteria.”
Knowing these evolutionary push-and-pull relationships could take the guesswork out of prescribing antibiotics, Santos-Lopez said.
In this experiment, when the biofilm evolved resistance to ciprofloxacin, it became defenseless against cephalosporins. The free-floating bacteria did not develop this same chink in their armor, even though they became 128 times more resistant to ciprofloxacin than the biofilm-grown bacteria.
According to study coauthor Michelle Scribner, a doctoral student in Cooper's lab, these findings highlight the importance of studying bacteria as they naturally occur, in biofilms.
“Biofilms are a more clinically relevant lifestyle,” Scribner said. “They're thought to be the primary mode of growth for bacteria living in the body. Most infections are caused by biofilms on surfaces.”
Additional contributors include co-first author Christopher Marshall, Ph.D., now at Marquette University, and Daniel Snyder, M.S., of Pitt.
This work was supported by a grant from the National Institutes of Health (U01AI124302-01).
To read this release online or share it, visit https:/
About the University of Pittsburgh Schools of the Health Sciences
The University of Pittsburgh Schools of the Health Sciences include the schools of Medicine, Nursing, Dental Medicine, Pharmacy, Health and Rehabilitation Sciences and the Graduate School of Public Health. The schools serve as the academic partner to the UPMC (University of Pittsburgh Medical Center). Together, their combined mission is to train tomorrow's health care specialists and biomedical scientists, engage in groundbreaking research that will advance understanding of the causes and treatments of disease and participate in the delivery of outstanding patient care. Since 1998, Pitt and its affiliated university faculty have ranked among the top 10 educational institutions in grant support from the National Institutes of Health. For additional information about the Schools of the Health Sciences, please visit http://www.
Contact: Erin Hare
Contact: Arvind Suresh
Alle Nachrichten aus der Kategorie: Life Sciences
Articles and reports from the Life Sciences area deal with applied and basic research into modern biology, chemistry and human medicine.
Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.
Smart sensors for future fast charging batteries
European project “Spartacus” launched Faster charging, longer stability of performance not only for electric vehicles but also for smartphones and other battery powered products. What still sounds like science fiction…
Small molecules control bacterial resistance to antibiotics
Antibiotics have revolutionized medicine by providing effective treatments for infectious diseases such as cholera. But the pathogens that cause disease are increasingly developing resistance to the antibiotics that are most…