Background Using the electroporation process, electrical pulses create pores that allow genetic material to permeate the bacterial membrane of electrocompetent cells. The limiting factor for this standard laboratory method is the efficiency by which DNA can be introduced into E. coli. Commercially available electrocompetent cells are being delivered frozen at about -70 to -80°C. The conventional wisdom is that storage at a higher temperature, for example at -20°C or higher, will result in a significant decrease in viability and transformation efficiency. Invention Inventors of Saarland University have established a new method and created a new bacterial strain to generate electrocompetent bacterial cells with a transformation efficiency of up to 5,6 x 1010, which can be handled at room temperature. This electrocompetent cells can be stored at 4°C without losing transformation efficiency. Also the new established protocol is able to improve the transformation efficiencies of commercially available bacterial cells.
Further information: PDF
Universität des Saarlandes Wissens- und Technologietransfer GmbH PatentVerwertungsAgentur der saarländischen Hochschulen
Phone: +49 (0)681/302-71302
Dipl.-Kfm. Axel Koch (MBA), Dr. Conny Clausen, Dr. Nicole Comtesse, Dr. Frank Döbrich
As Germany's association of technology- and patenttransfer agencies TechnologieAllianz e.V. is offering businesses access to the entire range of innovative research results of almost all German universities and numerous non-university research institutions. More than 2000 technology offers of 14 branches are beeing made accessable to businesses in order to assure your advance on the market. At www.technologieallianz.de a free, fast and non-bureaucratic access to all further offers of the German research landscape is offered to our members aiming to sucessfully transfer technologies.
email@example.com | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
What if, instead of turning up the thermostat, you could warm up with high-tech, flexible patches sewn into your clothes - while significantly reducing your...
A widely used diabetes medication combined with an antihypertensive drug specifically inhibits tumor growth – this was discovered by researchers from the University of Basel’s Biozentrum two years ago. In a follow-up study, recently published in “Cell Reports”, the scientists report that this drug cocktail induces cancer cell death by switching off their energy supply.
The widely used anti-diabetes drug metformin not only reduces blood sugar but also has an anti-cancer effect. However, the metformin dose commonly used in the...
A research team from the University of Zurich has developed a new drone that can retract its propeller arms in flight and make itself small to fit through narrow gaps and holes. This is particularly useful when searching for victims of natural disasters.
Inspecting a damaged building after an earthquake or during a fire is exactly the kind of job that human rescuers would like drones to do for them. A flying...
Over the last decade, there has been much excitement about the discovery, recognised by the Nobel Prize in Physics only two years ago, that there are two types...
What if a sensor sensing a thing could be part of the thing itself? Rice University engineers believe they have a two-dimensional solution to do just that.
Rice engineers led by materials scientists Pulickel Ajayan and Jun Lou have developed a method to make atom-flat sensors that seamlessly integrate with devices...
12.12.2018 | Event News
10.12.2018 | Event News
06.12.2018 | Event News
14.12.2018 | Ecology, The Environment and Conservation
13.12.2018 | Life Sciences
13.12.2018 | Physics and Astronomy