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Clot Lysis Assay ? measurement of fibrinolysis in all parts of the reaction

13.12.2006
At present, the fibrinolysis system is measured chromogenically via the enzyme activity of plasmin as a matter of routine. As the last reaction of fibrinolysis, i.e. the dissolution of fibrin strains, cannot be detected, this measurement system is only in part physiologi-cal.

The invention on hand provides a novel method rendering it possible to measure all steps of the fibrinolysis reaction, especially the dissolution of fibrin strain. Conventional microtiter plates containing micro thrombi are used in this novel clot lysis assay. Lysis is determined based on the regressing turbidity of the thrombus. Thereby, several different thrombolytic agents can be compared with each other. Both exogenic stimulation of plasmatic thrombolysis and intrinsic plasmatic thrombolysis of plasminogen activators can be measured. Experiments hitherto carried out with the novel clot lysis assay clearly indicate that different thrombolytic agents lead zu different clinical results, which most likely depend on endogenous thrombi.

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