cDNA-Synthesis from cells after microdissection
? Specially developed procedure for the analysis of individual eurons from fixed brain cross sections is applicable for a multitude of other cell types and tissues
? Contamination free isolation of individual cells from fixed tissue by means of laser microdissection and subsequently, the direct cDNA-ynthesis of the RNA of these cells ? The complex and risky step of RNA isolation is no longer necessary, because lysis, cDNA-Synthesis and, subsequently, PCR-reaction are combined in a reaction vessel and are conducted in a common buffer. ? Further steps, such as sample preparation, colouring, implementation of the incubation protocol and adherence to the temperature guidelines optimise the yield of RNA/cDNA ? Overall, the procedure is characterised by simple, quick and cost-efficient application
Further Information: PDF
TransMIT Gesellschaft für Technologietransfer mbH
Phone: +49 (0)641/943 64-12
Contact
Dr. Peter Stumpf
Media Contact
All latest news from the category: Technology Offerings
Newest articles
High-energy-density aqueous battery based on halogen multi-electron transfer
Traditional non-aqueous lithium-ion batteries have a high energy density, but their safety is compromised due to the flammable organic electrolytes they utilize. Aqueous batteries use water as the solvent for…
First-ever combined heart pump and pig kidney transplant
…gives new hope to patient with terminal illness. Surgeons at NYU Langone Health performed the first-ever combined mechanical heart pump and gene-edited pig kidney transplant surgery in a 54-year-old woman…
Biophysics: Testing how well biomarkers work
LMU researchers have developed a method to determine how reliably target proteins can be labeled using super-resolution fluorescence microscopy. Modern microscopy techniques make it possible to examine the inner workings…
