One-Plasmid Systems for Blue-light-regulated Gene Expression in Prokaryotes

<strong>Background</strong><br>

Protein expression systems are very widely used in the life sciences, biotechnology and medicine. Traditional strategies for recombinant protein expression involve the transformation of a gene expression vector into prokaryotic expression hosts and culturing the cells so that they express the desired protein. Using an inducible expression cassette the onset of the protein expression can be controlled by several inducer (f.e. chemical, temperature, cold).<br><br><strong>Technology</strong><br> We offer two compact plasmids for facile light regulated expression of target proteins in prokaryotic hosts. Dependent from the application, one plasmid can be used for light activated and one for light inactivated gene expression. Both plasmids are working without exogenous addition of chromophores or expensive chemical inducers and allow a specific, spatially- and temporally precise control of protein expression. A competitive advantage is the use of light as inducer especially for the production of recombinant proteins. Another advantage is the very precise control of protein expression which is very relevant for several research applications. By using standard chemical inducers a spatially and temporally precise control of protein expression is not possible.<br> <strong><br><br> <b>Benefits:</b> <ul> <li>Both plasmids are compact and ready-to-use</li> <li>No exogenous addition of chromophores or chemical inducers is required and thus reduced production costs</li> <li>System facilitates pulsed and/or automated induction of protein expression</li> <li>Specific, spatially- and temporally-precise protein expression</li> </ul><br><br> <p><strong>IP Rights</strong><br> European Patent Applic. (06/2009).<br> PCT Application (08/2010). <br><br> <strong>Patent Owner</strong><br> Humboldt-Universität zu Berlin <b>Find the technology offer and contact details here: <a href=”http://www.ipal.de/technologieangebote/11043″ target=”_blank”>http://www.ipal.de/technologieangebote/11043</a></b>

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