Novel genetic engineered yeast strains (Saccharomyces cerevisae) have been established that produce increased ethanol yield while simultaneously reduce the production of the unwanted by-product glycerol. It is a strong industrial interest to reduce the glycerol formation during glucose catabolism and thereby increasing ethanol yield, also because glycerol disturbs the distillation process. Past approaches to reduce glycerol formation based e.g. on the deletion of either one or the two genes gpd1 and gpd2 of glycerol-3-phosphate dehydrogenase (GPDH), which is the rate-controlling enzyme in the glycerol formation pathway of the yeast Saccharomyces cerevisae. These isoenzymes play also a crucial role in osmoregulation and redox balance. While single deletion of either gpd1 or gpd2 does not noticeble decrease glycerol production, the gpd1∆gpd2∆ double deletion strain produces no glycerol, however with the negative side effect that growth and ethanol production is abolished under anaerobic conditions and strongly reduced under aerobic conditions.<br><br> In the novel genetic engineering approaches, a) the Gpd1 enzyme activity is only partly reduced in a gpd2∆-deleted CEN-PK113 yeast strain background by replacing the strong natural gpd1 promotor by a weak TEF1 promotor mutant or b) both enzyme activities of GPD1 and GPD2 are partly reduced. The strains with reduced GPD1 and GPD2 activity show an increase in ethanol production by 2-5% and a reduction in glycerol formation by 61-88% compared to wild type and a slight better growth rate than the TEFmut:GPD1 gpd2∆-strain (ethanol increase: 6,3%; glycerol formation reduction by 64%).
The technology was developed at the Technische Universität Berlin.
Weitere Informationen: PDF
Tel.: +49 (0)30/2125-4820
Dr. Dirk Dantz
firstname.lastname@example.org | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
16.07.2018 | Physics and Astronomy
16.07.2018 | Life Sciences
16.07.2018 | Earth Sciences