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BioPlex-11: forensic method for typing of degraded and low copy DNA samples

23.02.2012
Over the past years in the field of forensic medicine creation of DNA profiles with biological traces has gained in importance enormously. Individual-related attribution of DNA results from so-called STR-loci (short tandem repeats), which contain specific genetic information on every individual. Multiplex-PCR-analyses are used for DNA-typing, though frequently only a fractional DNA-profile can be created, if the DNA is strongly degraded or if there is only low amount of DNA available.

The innovation comprises a method in which two PCR reactions (a sixplex and a fiveplex PCR) are combined into one large multiplex reaction using biotinylated and fluorescence-marked primers. Those primers enable the amplification and separation of overlapping PCR-fragments.

Further Information: PDF

IMG Innovations-Management GmbH
Phone: +49 (0)631/31668-50

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Dr. Klaus Kobek

info@technologieallianz.de | TechnologieAllianz e.V.
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http://www.technologieallianz.de/angebote.php?sort=sag&id=2651&lang=en
http://www.technologieallianz.de

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