Berkeley Researchers Create Unique Graphene Nanopores with Optical Antennas for DNA Sequencing
High-speed reading of the genetic code should get a boost with the creation of the world’s first graphene nanopores – pores measuring approximately 2 nanometers in diameter – that feature a “built-in” optical antenna.
Schematic drawing of graphene nanopore with self-integrated optical antenna (gold) that enhances the optical readout signal (red) of DNA as it passes through a graphene nanopore.
Researchers with Berkeley Lab and the University of California (UC) Berkeley have invented a simple, one-step process for producing these nanopores in a graphene membrane using the photothermal properties of gold nanorods.
“With our integrated graphene nanopore with plasmonic optical antenna, we can obtain direct optical DNA sequence detection,” says Luke Lee, the Arnold and Barbara Silverman Distinguished Professor at UC Berkeley.
Lee and Alex Zettl, a physicist who holds joint appointments with Berkeley Lab’s Materials Sciences Division and UC Berkeley’s Physics Department, were the leaders of a study in which a hot spot on a graphene membrane formed a nanopore with a self-integrated optical antenna. The hot spot was created by photon-to-heat conversion of a gold nanorod.
“We believe our approach opens new avenues for simultaneous electrical and optical nanopore DNA sequencing and for regulating DNA translocation,” says Zettl, who is also a member of the Kavli Energy Nanoscience Institute (Kavli ENSI).
Nanopore sequencing of DNA, in which DNA strands are threaded through nanoscale pores and read one letter at a time, has been touted for its ability to make DNA sequencing a faster and more routine procedure. Under today’s technology, the DNA letters are “read” by an electrical current passing through nanopores fabricated on a silicon chip.
Trying to read electrical signals from DNA passing through thousands of nanopores at once, however, can result in major bottlenecks. Adding an optical component to this readout would help eliminate such bottlenecks.
“Direct and enhanced optical signals are obtained at the junction of a nanopore and its optical antenna,” says Lee. “Simultaneously correlating this optical signal with the electrical signal from conventional nanopore sequencing provides an added dimension that would be an enormous advantage for high-throughput DNA readout.”
A key to the success of this effort is the single-step photothermal mechanism that enables the creation of graphene nanopores with self-aligned plasmonic optical antennas. The dimensions of the nanopores and the optical characteristics of the plasmonic antenna are tunable, with the antenna functioning as both optical signal transducer and enhancer.
The atomically thin nature of the graphene membrane makes it ideal for high resolution, high throughput, single-molecule DNA sequencing. DNA molecules can be labeled with fluorescent dyes so that each base-pair fluoresces at a signature intensity as it passes through the junction of the nanopore and its optical antenna.
“In addition, either the gold nanoplasmonic optical antenna or the graphene can be functionalized to be responsive to different base-pair combinations,” Lee says. “The gold plasmonic optical antenna can also be functionalized to enable the direct optical detection of RNA, proteins, protein-protein interactions, DNA-protein interactions, and other biological systems.”
The results of this study were reported in Nano Letters in a paper titled “Graphene Nanopore with a Self-Integrated Optical Antenna.” Lee is the corresponding author. Other co-authors in addition to Zettl were SungWoo Nam, Inhee Choi, Chi-cheng Fu, Kwanpyo Kim, SoonGweon Hong and Yeonho Choi.
This research was primarily supported by the DOE Office of Science.
Lynn Yarris | EurekAlert!
New type of low-energy nanolaser that shines in all directions
18.12.2018 | Eindhoven University of Technology
NASA research reveals Saturn is losing its rings at 'worst-case-scenario' rate
18.12.2018 | NASA/Goddard Space Flight Center
Researchers from the University of Basel have reported a new method that allows the physical state of just a few atoms or molecules within a network to be controlled. It is based on the spontaneous self-organization of molecules into extensive networks with pores about one nanometer in size. In the journal ‘small’, the physicists reported on their investigations, which could be of particular importance for the development of new storage devices.
Around the world, researchers are attempting to shrink data storage devices to achieve as large a storage capacity in as small a space as possible. In almost...
The more objects we make "smart," from watches to entire buildings, the greater the need for these devices to store and retrieve massive amounts of data quickly without consuming too much power.
Millions of new memory cells could be part of a computer chip and provide that speed and energy savings, thanks to the discovery of a previously unobserved...
What if, instead of turning up the thermostat, you could warm up with high-tech, flexible patches sewn into your clothes - while significantly reducing your...
A widely used diabetes medication combined with an antihypertensive drug specifically inhibits tumor growth – this was discovered by researchers from the University of Basel’s Biozentrum two years ago. In a follow-up study, recently published in “Cell Reports”, the scientists report that this drug cocktail induces cancer cell death by switching off their energy supply.
The widely used anti-diabetes drug metformin not only reduces blood sugar but also has an anti-cancer effect. However, the metformin dose commonly used in the...
A research team from the University of Zurich has developed a new drone that can retract its propeller arms in flight and make itself small to fit through narrow gaps and holes. This is particularly useful when searching for victims of natural disasters.
Inspecting a damaged building after an earthquake or during a fire is exactly the kind of job that human rescuers would like drones to do for them. A flying...
12.12.2018 | Event News
10.12.2018 | Event News
06.12.2018 | Event News
18.12.2018 | Materials Sciences
18.12.2018 | Physics and Astronomy
18.12.2018 | Physics and Astronomy