The research is published in SPIE's Journal of Biomedical Optics
A paper published in the Journal of Biomedical Optics (JBO), "Imaging hydroxyapatite in sub-retinal pigment epithelial deposits by fluorescence lifetime imaging microscopy with tetracycline staining," demonstrates a potential new diagnostic option for catching a degenerative eye disease in its earliest stages.
Tiny deposits of lipids, proteins, and minerals, sometimes known as drusen, can collect under the retina. They may indicate a person's risk of developing age-related macular degeneration (AMD), so enhanced and early detection is a current clinical need.
The initial findings presented in the paper show a new way to achieve molecular contrast based upon sub-retinal mineral deposits. The approach uses a well-known property of the tetracycline-family of antibiotics - their propensity to stain teeth and bones - making visible the smallest of mineral deposits.
Utilizing human cadaver retinas containing drusen, the researchers used fluorescence lifetime imaging microscopy (FLIM) to measure the light emission from tetracycline staining within those ocular mineral deposits.
According to JBO Editor-in-Chief, SPIE Fellow, and MacLean Professor of Engineering at Dartmouth Brian W. Pogue, the novel use of FLIM to harness the properties of a common antibiotic marks an exciting approach to molecular imaging in the eye, and opens up the potential for developing a diagnostic test for early transition to eye disease:
"Eye diseases are typically diagnosed by shape, blood flow, or morphology changes in the retina, but this fluorescence test could be more sensitive because the signal is highly specific. It can be used to detect very small molecular mineral deposits that cannot be seen well otherwise and detect them early in the disease process. Since this is a first study, a lot more work needs to be done to make this a real human diagnostic test, but the concept that a common antibiotic could be used in this way, to reveal small mineral deposits, is a very important fundamental discovery."
The article authors are Henryk Szmacinski, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland; Kavita Hegde, Department of Natural Sciences, Coppin State University, Baltimore, Maryland; Hui-Hui Zeng, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine; Katayoun Eslami, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine; Adam Puche, Department of Anatomy and Neuroscience, University of Maryland School of Medicine; Imre Lengyel, Wellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast, UK; and Richard B. Thompson, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine.
JBO, an open-access journal, is published by SPIE in the SPIE Digital Library, which contains more than 500,000 publications from SPIE journals, proceedings, and books, with approximately 18,000 new research papers added each year.
SPIE is the international society for optics and photonics, an educational not-for-profit organization founded in 1955 to advance light-based science, engineering, and technology. The Society serves more than 255,000 constituents from 183 countries, offering conferences and their published proceedings, continuing education, books, journals, and the SPIE Digital Library. In 2019, SPIE provided more than $5.6 million in community support including scholarships and awards, outreach and advocacy programs, travel grants, public policy, and educational resources. http://www.
Daneet Steffens | EurekAlert!
Scientists use nanoparticle-delivered gene therapy to inhibit blinding eye disease in rodents
08.07.2020 | Johns Hopkins Medicine
Deconstructing glioblastoma complexity reveals its pattern of development
08.07.2020 | McGill University
Kiel physics team observed extremely fast electronic changes in real time in a special material class
In physics, they are currently the subject of intensive research; in electronics, they could enable completely new functions. So-called topological materials...
Solar cells based on perovskite compounds could soon make electricity generation from sunlight even more efficient and cheaper. The laboratory efficiency of these perovskite solar cells already exceeds that of the well-known silicon solar cells. An international team led by Stefan Weber from the Max Planck Institute for Polymer Research (MPI-P) in Mainz has found microscopic structures in perovskite crystals that can guide the charge transport in the solar cell. Clever alignment of these "electron highways" could make perovskite solar cells even more powerful.
Solar cells convert sunlight into electricity. During this process, the electrons of the material inside the cell absorb the energy of the light....
Empa researchers have succeeded in applying aerogels to microelectronics: Aerogels based on cellulose nanofibers can effectively shield electromagnetic radiation over a wide frequency range – and they are unrivalled in terms of weight.
Electric motors and electronic devices generate electromagnetic fields that sometimes have to be shielded in order not to affect neighboring electronic...
A promising operating mode for the plasma of a future power plant has been developed at the ASDEX Upgrade fusion device at Max Planck Institute for Plasma...
Live event – July 1, 2020 - 11:00 to 11:45 (CET)
"Automation in Aerospace Industry @ Fraunhofer IFAM"
The Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM l Stade is presenting its forward-looking R&D portfolio for the first time at...
07.07.2020 | Event News
02.07.2020 | Event News
19.05.2020 | Event News
08.07.2020 | Physics and Astronomy
08.07.2020 | Agricultural and Forestry Science
08.07.2020 | Materials Sciences