Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Stratification of the severity of Non Alcoholic Fatty Liver disease

09.12.2015

A European Consortium led by researcher James Adjaye, medical faculty of Heinrich Heine University-Duesseldorf and former Max-Planck researcher, identify a means of predicting the severity of Non Alcoholic Fatty Liver Disease (NAFLD) in a small cohort of patients. With an estimated prevalence of about 40 % in Europe and 50 % in the general world population, NAFLD, also referred to as steatosis, is a major public health issue highly related to our sedentary life style and high-fat diets. The prevalence is expected to increase rapidly leading to an epidemic status. The article was recently published in Nature Press - Scientific Data 2:150068 doi: 10.1038/sdata.2015.68 (2015).

It has been estimated in Europe that approximately 26 % or higher health costs account for NAFLD patients.NAFLD is a typical metabolic disease associated with obesity, insulin resistance and type 2 diabetes.

Abnormal fat deposits develop in patients’ livers. In general steatosis is without complications however associated with an increased risk of heart attacks and strokes. Furthermore, steatosis can progress to steatohepatitis and in some cases to cirrhosis and liver cancer.

There are also genetic mutations associations with the disease, for example within genes such as PNPLA3 and TM6SF2. Taken together, NAFLD is caused by a complex interplay of genetic and environmental factors.

Therefore to gain insights into disease causing mechanisms James Adjaye co-ordinated the livSYSiPS consortium, which was co-funded by the German Federal Ministry of Education and Research (BMBF), a partner of the ERASysBio+ initiative supported under the EU ERA-NET Plus scheme in FP7. The ERASysBio+ initiative aimed at advancing the progress of systems biology research in Europe. The multi-national consortium with research groups from Germany, UK, Italy and Austria employed a systems biology approach to investigate the etiology of NAFLD.

The underlying study discovered higher amounts of the gene PLIN2 in NAFLD patients. PLIN2 is involved in the aggregation of the fat deposits in the liver. Mice without this protein do not become fat even when overfed with a high-fat diet.

Additional findings in blood serum of these patients include differences in the levels of the appetite-controlling hormone LEPTIN, ADIPONECTIN and amino acids which control the break-down of fat. Furthermore, changes in the complex signaling cascades of insulin were detected which is the main regulation mechanism of glucose and fat balance.

These distinctions are an encouraging basis for further research encompassing a much larger cohort of patients with the potential to find novel means for diagnosing NAFLD and eventually new therapies.

Essential for this goal is good quality systems biology-based datasets and models and the sharing of data between researchers to catalyze scientific progress. Wasco Wruck, Bioinformatician and first author of the study, says:

”From our multi-disciplinary measurements we got amazing datasets from which we could draw interesting findings and hypothesis about NAFLD. We need further patient cohorts and experiments to confirm our initial results and to improve our systems biology models. In order to allow other researchers to work on this valuable data we deposited it in public repositories.”

To gain further insights to the cause of the disease at the level of individuals, which is the new era of personalized medicine, James Adjaye’s team are now generating induced pluripotent stem cells (iPSCs) from skin cells from these patients and matching healthy controls. These patient-specific iPSCs are then turned into hepatocytes which are cells within the liver with the view of mimicking the patient’s liver metabolism in a dish.

To induce steatosis we add excess fat (e.g. oleic acid) to these cells and then analyse changes in gene, protein and metabolite levels as we originally did with the original liver biopsy and blood serum samples. Our goal now is to see if our “disease in a dish” model mirrors that seen in the patient, define new biomarkers of NAFLD and identify pathways as putative drug targets that can be used to complement current therapies for NAFLD says James Adjaye who is the senior author of the current publication.

Original Publikation:
Wasco Wruck, Karl Kashofer, Samrina Rehman, Andriani Daskalaki, Daniela Berg, Ewa Gralka, Justyna Jozefczuk, Katharina Drews, Vikash Pandey, Christian Regenbrecht, Christoph Wierling, Paola Turano, Ulrike Korf, Kurt Zatloukal, Hans Lehrach, Hans Westerhoff, and James Adjaye
Multi-omic profiles of human non-alcoholic fatty liver disease tissue highlight heterogenic phenotypes
Nature Press - Scientific Data 2:150068 doi: 10.1038/sdata.2015.68 (2015)

Weitere Informationen:

http://www.erasysbio.net/index.php?index=264 ERASysBio+ initiative
http://www.uniklinik-duesseldorf.de/isrm Institut für Stammzellforschung und Regenerative Medizin, Duesseldorf University

Susanne Dopheide | idw - Informationsdienst Wissenschaft

More articles from Health and Medicine:

nachricht The genes are not to blame
20.07.2018 | Technische Universität München

nachricht Targeting headaches and tumors with nano-submarines
20.07.2018 | Universitätsmedizin der Johannes Gutenberg-Universität Mainz

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Future electronic components to be printed like newspapers

A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.

The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

A smart safe rechargeable zinc ion battery based on sol-gel transition electrolytes

20.07.2018 | Power and Electrical Engineering

Reversing cause and effect is no trouble for quantum computers

20.07.2018 | Information Technology

Princeton-UPenn research team finds physics treasure hidden in a wallpaper pattern

20.07.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>