Kansas State University diagnosticians are helping the cattle industry save millions of dollars each year by developing earlier and accurate detection of E. coli.
Lance Noll, master's student in veterinary biomedical science, Greensburg; T.G. Nagaraja, university distinguished professor of diagnostic medicine and pathobiology; and Jianfa Bai, assistant professor in the Kansas State Veterinary Diagnostic Laboratory, are leading a project to improve techniques for detecting pathogenic Shiga toxin-producing E. coli O157:H7. A U.S. Department of Agriculture Coordinated Agriculture Project grant is funding the work.
The researchers are part of a College of Veterinary Medicine team studying preharvest food safety in beef cattle. Noll has developed and validated a molecular assay that can detect and quantify major genes specific for E. coli O157.
"Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply," Noll said.
The newly developed test is a molecular assay, or polymerase chain reaction, that detects bacteria based on genetic sequences, which are the bacteria's "fingerprints," Nagaraja said. The test is rapid and less labor-intensive than existing detection methods. The method can be automated and test many samples in a short period of time.
The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities.
"The novelty of this test is that it targets four genes," Nagaraja said. "We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces."
To develop the diagnostic test, Noll and Nagaraja worked with two Kansas State University molecular biologists: Xiaorong Shi, research assistant of diagnostic medicine and pathobiology, and Bai.
"Beef cattle production is a major industry in Kansas and Kansas State University has a rich tradition in the research of beef cattle production and beef safety," Noll said. "As a graduate student in veterinary biomedical sciences, I am proud to be a member of a multidisciplinary team in the College of Veterinary Medicine that aims to make beef a safe product for the consumers."
Noll was named a winner at the 11th annual Capitol Graduate Research Summit this spring for his research project and poster, "A four-plex real-time PCR assay for the detection and quantification of Escherichia coli O157 in cattle feces."
T.G. Nagaraja | Eurek Alert!
Mechanism Discovered to Activate the Immune System against Bacteria and Regulate the Microbiome
13.02.2019 | Universitätsklinikum Tübingen
For the first time, an international team of scientists based in Regensburg, Germany, has recorded the orbitals of single molecules in different charge states in a novel type of microscopy. The research findings are published under the title “Mapping orbital changes upon electron transfer with tunneling microscopy on insulators” in the prestigious journal “Nature”.
The building blocks of matter surrounding us are atoms and molecules. The properties of that matter, however, are often not set by these building blocks...
Scientists at the University of Konstanz identify fierce competition between the human immune system and bacterial pathogens
Cell biologists from the University of Konstanz shed light on a recent evolutionary process in the human immune system and publish their findings in the...
Laser physicists have taken snapshots of carbon molecules C₆₀ showing how they transform in intense infrared light
When carbon molecules C₆₀ are exposed to an intense infrared light, they change their ball-like structure to a more elongated version. This has now been...
The so-called Abelian sandpile model has been studied by scientists for more than 30 years to better understand a physical phenomenon called self-organized...
Physicists from the University of Basel have developed a new method to examine the elasticity and binding properties of DNA molecules on a surface at extremely low temperatures. With a combination of cryo-force spectroscopy and computer simulations, they were able to show that DNA molecules behave like a chain of small coil springs. The researchers reported their findings in Nature Communications.
DNA is not only a popular research topic because it contains the blueprint for life – it can also be used to produce tiny components for technical applications.
11.02.2019 | Event News
30.01.2019 | Event News
16.01.2019 | Event News
15.02.2019 | Physics and Astronomy
15.02.2019 | Physics and Astronomy
15.02.2019 | Life Sciences