They discovered that a specific surface receptor (NB1) mediates the expression of a molecule (autoantigen) on the surface of certain white blood cells, where the immune system recognizes and binds it. This reaction starts the inflammation process. Thus, NB1 is a potential drug target for ANCA-associated vasculitis, as Dr. Sibylle von Vietinghoff (FVK), Professor Friedrich Luft (FVK, MDC) and Professor Ralph Kettritz (FVK) report in the American journal Blood (Vol. 109, No. 10, pp. 4487-4493, May 15, 2007)*.
Autoimmune vasculitis is a broad term encompassing a group of disorders characterized by blood vessel inflammation. It is triggered by antibodies formed by the immune system which are no longer able to distinguish between “self” and “non-self”. The antibodies target the most common white blood cells, the neutrophil granulocytes, or more precisely an enzyme that is normally found in the cell plasma of these blood cells, proteinase 3 (PR3).
Neutrophil granulocytes are part of the immune system, and their primary function is to attack viruses and bacteria. In the cell plasma of all neutrophil granulocytes, the enzyme proteinase 3 (PR3) is present. PR3 plays a role in one form of vasculitis because it elicits the formation of autoantibodies. These antibodies are therefore called ANCA (antineutrophil cytoplasmatic autoantibodies).
However, ANCA autoantibodies only attack the white blood cells which “display” the enzyme PR3 on their surface. The blood cells which retain the enzyme inside the cell away from the cell surface avoid the attack of the autoantibodies. The binding of the ANCA antibodies to the PR3 localized on the cell membrane activates the white blood cells, which thereupon attack the body’s own structures and trigger the inflammation processes in the blood vessels. The resulting inflammation can affect any and all organs and become life-threatening.
Prior to this study it was not known how the enzyme is presented on the membrane surface of the blood cells. Dr. von Vietinghoff and her colleagues in Berlin-Buch were able to show that it has a “helper” presenting it on the surface of the blood cells: the membrane anchor protein NB1 (CD177). For their study they tested and compared blood samples obtained from 200 healthy volunteer donors and patients with ANCA vasculitis. The researchers discovered that the enzyme PR3 and the receptor NB1 were more often found on the surfaces of blood cells in patients with ANCA vasculitis. Using molecular techniques the researchers were able to prove that NB1 is necessary for PR3 membrane presentation.
Aim – Development of a specific therapy
NB1 therefore appears to be a promising therapeutic target, according to Sibylle von Vietinghoff, Ralph Kettritz and Friedrich Luft. Although today there are treatments for vasculitis and related disorders such as Wegener’s granulomatosis, a rare but serious disease which affects all body organs including the kidneys, currently used drugs suppress the entire immune system. The disadvantage is that this makes patients more vulnerable to infection.
With unspecific immune therapy and dialysis, the kidneys of the affected patients often recover their function. “It is one of the few kidney diseases in which patients are able to come off dialysis,” Ralph Kettritz said. But a risk of recurrence always remains. If NB1 could be inhibited and PR3 expression on the blood cell surfaces prevented, vasculitis could be better controlled or even cured and recurrences avoided. “That is why our findings provide hope for patients with vasculitis,” he said. “A specific therapy approach based on our study may hold the key to overcoming the disease.”
Barbara Bachtler | alfa
Why might reading make myopic?
18.07.2018 | Universitätsklinikum Tübingen
Unique brain 'fingerprint' can predict drug effectiveness
11.07.2018 | McGill University
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
18.07.2018 | Life Sciences
18.07.2018 | Materials Sciences
18.07.2018 | Health and Medicine