Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The diagnosis of tumours in children

28.06.2005


Determining if there exist genetic alterations that can be associated with the diagnosis and prognosis of neuroblastic tumours, responsible for 15% of child deaths due to cancer, was the aim of the Paula Lázcoz Ripoll’s PhD thesis which she recently defended at the Public University of Navarra. The work was entitled: Molecular diagnosis of neuroblastic tumours: genetic profile and analysis of tumour suppressor genes.



Genetic alterations

Neuroblastic tumours are malignant extracraneal tumours most frequent in infancy: they affect 1 in every 8,000 children. Amongst these tumours are ganglioneuromas, ganglioneuroblastomas and neuroblastomas, the latter being the most malignant. According to the authoress of the thesis, in some cases, treatment is not required and they revert to their original state. But there are other neuroblastomas that are more aggressive and require an excision of the tumour and the subsequent treatment with radiotherapy and chemotherapy, although with scant percentage of success.


In this context, Ms Paula Lazcoz put forward as the objective of her PhD an attempt, from a molecular pathology perspective, at the diagnosis and prognosis of neuroblastic tumours. To this end, 45 neuroblastic tumours were analysed and 12 cellular lines of neuroblastoma, i.e. cells extracted from this type of tumour and maintained in culture.

Also, eight tumour suppressor genes that appear altered in numerous types of cancer were studied. In concrete, the genes involved are PTEN, DMBT1, MGMT, FGFR2, MXI1, RASSF1A, NORE1A, BLU and CASP8. According to Paula Lazcoz, in normal conditions, the tumour suppressor genes avoid a normal cell transforming into a tumorous cell and thus giving rise to a tumour, and so the alteration of any of these genes could be involved in the genesis of a tumour.

In this way, the corresponding DNA was obtained from the tumours and the indicated cellular lines, in order to subsequently determine the frequency of homozygotic delections, loss of heterozygosity, instability of microsatellites, hypermethylation of the promoter and the level of genetic expression of the previously mentioned genes, all of these being mechanisms that can inactivate the tumour suppressor genes and, thus, favour the formation of the tumour. The techniques used in order to carry out this analysis were differential PCR, standard PCR, MSP and RT-PCR. The profile of gains and losses of genetic material at a global genome level was also studied by means of CGH.

Once the genetic alterations are determined, it was observed that no statistically significant relationship existed between the genetic alterations found and the clinical-pathological data of the tumours (age, sex, tumour location, etc). According to the authoress, these results could be due to the fact that the sample, given its size, was not very representative thus begging the question that, with a wider sample, more significant data would be obtained.

Nevertheless, a statistically significant relationship was in fact observed (p<0.05) between the hypermethylation of the RASSF1A and CASP8 genes, which might suggest the need to alter simultaneously two routes of carcinogenetic control in order to obtain a neuroblastic tumour.

Garazi Andonegi | alfa
Further information:
http://www.elhuyar.com
http://www.basqueresearch.com

More articles from Health and Medicine:

nachricht Using fragment-based approaches to discover new antibiotics
21.06.2018 | SLAS (Society for Laboratory Automation and Screening)

nachricht Scientists learn more about how gene linked to autism affects brain
19.06.2018 | Cincinnati Children's Hospital Medical Center

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Temperature-controlled fiber-optic light source with liquid core

In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.

Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...

Im Focus: Overdosing on Calcium

Nano crystals impact stem cell fate during bone formation

Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

Im Focus: Sharp images with flexible fibers

An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.

Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

Graphene assembled film shows higher thermal conductivity than graphite film

22.06.2018 | Materials Sciences

Fast rising bedrock below West Antarctica reveals an extremely fluid Earth mantle

22.06.2018 | Earth Sciences

Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View

22.06.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>