Case Western Reserve University School of Medicine finding holds promise for repairing damaged hearing
Hearing is made possible when hair bundles protruding from the tops of hair cells capture the energy of sound waves, converting them into electrical signals that stimulate the auditory nerve to the brain. These hair bundles are made up of individual hair-like projections, or stereocilia, which sway in unison with other stereocilia, and remain permanently with us throughout our lives. Extremely loud noise can force whipsaw motion of the stereocilia, causing them to be damaged and the resulting hearing loss to be permanent. The prevailing theory had been that these hair bundles were made up of cellular scaffolding proteins that do not change or circulate.
Each single hair bundle within the live zebrafish ear contains fascin 2b fused with green fluorescent protein. In this time coarse, the fascin 2b molecules migrate to other regions of the bundle within 220 seconds.
Credit: Cell Reports: Adapted from Figure 1 in "The Stereociliary Paracrystal is a Dynamic Cytoskeletal Scaffold In Vivo"
Scientists at Case Western Reserve University School of Medicine have discovered that the movement of protein within hair cells of the inner ear shows signs of a repair and renewal mechanism.
The investigator's findings will be showcased as the cover paper in the Nov. 17 edition of Cell Reports and are available now in the online edition of the journal. The discovery paves the way for one day therapeutically manipulating the movement of these proteins in a way that could reverse hearing loss, a condition that affects approximately 15 percent of Americans between the ages of 20 and 69.
"What was surprising in our research with zebrafish is that proteins move so rapidly, implying that protein movement may be required to maintain the integrity of hair bundles in the inner ear," said senior author Brian McDermott, PhD, associate professor of Otolaryngology-Head and Neck Surgery, Case Western Reserve University School of Medicine. "Our research tells us that constant movement, replacement and adjustment among proteins in the inner ear's hair bundles serve a maintenance and even repair function."
In their research, School of Medicine investigators discovered that proteins in the hair bundle move at surprisingly fast rates, and this movement could explain why hair bundles last a lifetime. The researchers used a confocal microscope to examine the movement in real time of these proteins within the inner ears of baby zebrafish. The zebrafish at this early state of development are completely transparent, making it possible to view internal organs without dissection, including ear structure.
"We made movies of the secret inner workings of the hair bundle in a live animal, and what is happening in the ear is amazing and unexpected," McDermott said.
The researchers discovered that vital proteins, actin and myosin, move at a leisurely pace over the course of hours. On the other hand, fascin 2b moves incredibly fast, in seconds. This research proved that the internal structure of stereocilia is dynamic, that the proteins move frequently rather than being mostly stationary.
The speed with which fascin 2b moves may facilitate repair of breaks in the actin cables of the hair bundle when the ear is exposed to loud noise. Sound enters the ear at remarkable speeds, causing intense vibrations and back-and-forth movement of the hair bundle at extremely high rates. Investigators found that what appears to make the hair bundle so durable in the face of such forces is the rate of protein turnover, which enables renewal of the hair bundle.
"It was once thought that most everything within the stereocilia was relatively immobile and static," McDermott said. "We found that the constant, dynamic movement likely contributes to the permanency of the hair bundle structure to last a lifetime, or 70 to 90 years in human terms."
As a next research step, McDermott's team will determine how the hair bundle may repair itself with such protein movement. The process likely involves fascin 2b but may also involve other proteins. They will continue to visualize this process fully in the transparent zebrafish ear.
"No one has shown that stereocilia heal themselves, so next we will find out if there is a repair mechanism," he said. "People go deaf from damage to stereocilia in the inner ear. If we figure out ways of manipulating protein dynamics within stereocilia to heal this damage, we would likely be able to diminish hearing loss in humans."
Joining McDermott in this research were lead coauthors Philsang Hwang and Shih-Wei Chou and contributing author Zongwei Chen.
This research was funded by National Institutes of Health grants DC009437 and S10RR017980 and by the Center for Clinical Research and Technology at University Hospitals Case Medical Center.
Jeannette Spalding | EurekAlert!
Why might reading make myopic?
18.07.2018 | Universitätsklinikum Tübingen
Unique brain 'fingerprint' can predict drug effectiveness
11.07.2018 | McGill University
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
18.07.2018 | Materials Sciences
18.07.2018 | Life Sciences
18.07.2018 | Health and Medicine