Currently licensed live attenuated canine distemper vaccines are highly effective. However, they retain the potential to cause disease in very susceptible species. Researchers at Paul-Ehrlich-Institut and Thomas Jefferson University have generated an inactivated candidate vaccine that induces protective immunity against both rabies and canine distemper and has an excellent safety profile. The candidate vaccine consists of a mix of two recombinant inactivated rabies viruses that carry either one of the two canine distemper glycoproteins in addition to its own glycoprotein.The results are published in the online edition of Journal of Virology 1 February 2016.
Rabies and canine distemper are among the most dangerous and lethal viral diseases afflicting carnivores. Rabies can also be transmitted to humans via bite from rabies-infected animals. It attacks the nervous system and can be deadly if not treated immediately.
Cells infected with canine distemper virus fuse to neighboring uninfected cells to form syncytia. Antibodies against canine distemper virus are used to stain the infected cells with a red dye
Rabies vaccines contain inactivated virus particles and have an excellent stability and safety profile. For vaccination against canine distemper virus, which causes a measles-like disease in its hosts, live-attenuated viruses are used. These vaccine viruses replicate in the vaccinated animals to a certain extent and can lead to severe disease in highly susceptible species.
Prof. Dr. Veronika von Messling's group at the Veterinary Medicine Division, Paul-Ehrlich-Institut, in collaboration with Dr. Matthias Schnell's group at Thomas Jefferson University genetically engineered the rabies virus vaccine strain to carry one of the canine distemper virus glycoproteins in addition to its own glycoprotein. The protective immune response is directed against these proteins. Candidate vaccines were then produced following the purification and inactivation protocols used for rabies vaccines.
The researchers first demonstrated that a single shot was sufficient to elicit protective rabies antibody titers in ferrets. However, protection against canine distemper was only achieved when animals were immunized with a mix of viruses carrying both canine distemper virus glycoproteins. The researchers conclude that immune responses against both glycoproteins are necessary to protect from canine distemper.
"Our rabies-based vector is a promising platform for the development of new morbillivirus vaccines" explains von Messling. "It eliminates the risk for vaccine-induced disease and at the same time greatly increases the temperature stability compared to live-attenuated morbillivirus vaccines."
Da Fontoura Budaszewski R, Hudacek A, Sawatsky B, Krämer B, Xiangping Y, Schnell MJ, von Messling V (2017): Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity Against Both Pathogens.
J Virol Feb 01 [Epub ahead of print].
The Paul-Ehrlich-Institut, the Federal Institute for Vaccines and Biomedicines, in Langen near Frankfurt/Main is a senior federal authority reporting to the Federal Ministry of Health (Bundesministerium für Gesundheit, BMG). It is responsible for the research, assessment, and marketing authorisation of biomedicines for human use and immunological veterinary medicinal products. Its remit also includes the authorisation of clinical trials and pharmacovigilance, i.e. recording and evaluation of potential adverse effects.
Other duties of the institute include official batch control, scientific advice and inspections. In-house experimental research in the field of biomedicines and life science form an indispensable basis for the manifold tasks performed at the institute.
The Paul-Ehrlich-Institut, with its roughly 800 members of staff, also has advisory functions nationally (federal government, federal states (Länder)), and internationally (World Health Organisation, European Medicines Agency, European Commission, Council of Europe etc.).
http://jvi.asm.org/content/early/2017/01/26/JVI.02077-16.abstract - Abstract of the publicaton
http://www.pei.de/EN/information/journalists-press/press-releases/2017/03-inacti... - this press release on the Paul-Ehrlich-Institut Website
Dr. Susanne Stöcker | idw - Informationsdienst Wissenschaft
Inhaling air pollution-like irritant alters defensive heart-lung reflex for hypertension
19.06.2019 | University of South Florida (USF Innovation)
Nitric oxide-scavenging hydrogel developed for rheumatoid arthritis treatment
06.06.2019 | Pohang University of Science & Technology (POSTECH)
From June 25th to 27th 2019, the Fraunhofer Institute for Digital Media Technology IDMT in Ilmenau (Germany) will be presenting a new solution for acoustic quality inspection allowing contact-free, non-destructive testing of manufactured parts and components. The method which has reached Technology Readiness Level 6 already, is currently being successfully tested in practical use together with a number of industrial partners.
Reducing machine downtime, manufacturing defects, and excessive scrap
The quality of additively manufactured components depends not only on the manufacturing process, but also on the inline process control. The process control ensures a reliable coating process because it detects deviations from the target geometry immediately. At LASER World of PHOTONICS 2019, the Fraunhofer Institute for Laser Technology ILT will be demonstrating how well bi-directional sensor technology can already be used for Laser Material Deposition (LMD) in combination with commercial optics at booth A2.431.
Fraunhofer ILT has been developing optical sensor technology specifically for production measurement technology for around 10 years. In particular, its »bd-1«...
The well-known representation of chemical elements is just one example of how objects can be arranged and classified
The periodic table of elements that most chemistry books depict is only one special case. This tabular overview of the chemical elements, which goes back to...
Light can be used not only to measure materials’ properties, but also to change them. Especially interesting are those cases in which the function of a material can be modified, such as its ability to conduct electricity or to store information in its magnetic state. A team led by Andrea Cavalleri from the Max Planck Institute for the Structure and Dynamics of Matter in Hamburg used terahertz frequency light pulses to transform a non-ferroelectric material into a ferroelectric one.
Ferroelectricity is a state in which the constituent lattice “looks” in one specific direction, forming a macroscopic electrical polarisation. The ability to...
Researchers at TU Graz calculate the most accurate gravity field determination of the Earth using 1.16 billion satellite measurements. This yields valuable knowledge for climate research.
The Earth’s gravity fluctuates from place to place. Geodesists use this phenomenon to observe geodynamic and climatological processes. Using...
24.06.2019 | Event News
29.04.2019 | Event News
17.04.2019 | Event News
24.06.2019 | Event News
24.06.2019 | Agricultural and Forestry Science
24.06.2019 | Life Sciences