Two hearts, said Keats, can beat as one, but a study led by Weizmann Institute scientists in collaboration with researchers from the University of Pennsylvania shows that sometimes a single heart muscle cell can beat as more than two dozen.
The findings, reported recently in Nature Communications, provide an extremely detailed glimpse into the mechanisms behind normal and irregular heart muscle cell contractions. The study may help define the limitations of existing therapies for abnormal heartbeat and, in the future, suggest ways of designing new ones.
Weizmann Institute of Science
A chicken heart-muscle cell under a fluorescent microscope; the filaments consist of repeated subunits (bright dotted lines). The schematic representation shows three neighboring filaments; the black lines are the boundaries of their subunits, such that the lower filament is aligned with the middle one, while the upper one is not.
Each heart muscle cell consists of numerous parallel filaments comprising repeated subunits. When the heart beats, each individual filament contracts to produce muscle cell contractions.
Optimally, all the filaments should contract in a synchronized manner, thus ensuring the greatest amplitude of contraction for each muscle cell and ultimately, the strongest and most effective beating of the entire heart. However, a new theoretical model proposed and analyzed by Prof. Samuel Safran and postdoctoral fellow Dr. Kinjal Dasbiswas of the Weizmann Institute’s Department of Materials and Interfaces suggests that the filaments contract together only when their subunits, and subunit boundaries, are aligned with one another.
Since such alignment usually only happens among a limited number of neighboring filaments, these contract together as a bundle -- however, each such bundle contracts out of phase with others. Therefore, a heart cell does not necessarily beat as a single uniform entity; rather, the number of different beating entities in the cell depends on the bundle number, which may reach more than two dozen.
The theory, which uses the methods of statistical physics, further predicted that the alignment of the filaments in the heart muscle cell depends on the cell’s physical environment, and more specifically on the elasticity of the supporting structure (called the extracellular matrix). The alignment is best when this structure is not too soft and not too rigid. The prediction took into consideration various forces operating on the microscale, particularly mechanical forces that are exerted on each filament subunit by neighboring filaments via the extracellular matrix.
By assuming that only structurally aligned filaments beat together, the Weizmann theorists were able to quantitatively explain experimental findings by their collaborators from the University of Pennsylvania (aka Penn), Prof. Dennis Discher and Dr. Stephanie Majkut. In the experiments, the Penn scientists had placed embryonic heart cells from chicks on support surfaces of varying stiffness, and found that two strikingly different properties – the structural alignment of the filaments and the beating strength of the cell – depended on the rigidity of the supporting surface.
By providing a theoretical basis for these experiments, the Weizmann model may help explain how filaments become aligned in heart muscle cells during embryonic development, and how their arrangement correlates with the muscle function in the adult heart.
This correlation suggests that the current means of treating irregular heartbeat may be limited to a certain extent by the structural order of heart muscle filaments – however, the new understanding may one day help design improved treatments for heart disease. For example, in the future, if new heart cells are grown to replace diseased ones, their growth environment may be manipulated so that their structure is well ordered and, to paraphrase Keats, all their filaments beat as one.
Prof. Samuel Safran’s research is supported by the Gerhardt M.J. Schmidt Minerva Center on Supramolecular Architectures, which he heads; the US-Israel Binational Science Foundation; the Israel Science Foundation; Antonio and Noga Villalon, Winnetka, IL; the Clore Center for Biological Physics; the Kimmelman Center for Structural Biology; and the Kimmel Stem Cell Research Institute. Prof. Safran is the incumbent of the Fern and Manfred Steinfeld Professorial Chair.
Dr. Kinjal Dasbiswas’s research is supported by a fellowship from the Council of Higher Education.
Director, Science Content
Jennifer Manning | newswise
The genes are not to blame
20.07.2018 | Technische Universität München
Targeting headaches and tumors with nano-submarines
20.07.2018 | Universitätsmedizin der Johannes Gutenberg-Universität Mainz
A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.
The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
20.07.2018 | Materials Sciences
20.07.2018 | Physics and Astronomy
20.07.2018 | Materials Sciences